Western Blot (WB)
(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human His tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1: OTUB2-His tag Transfected 293T cell lysate;;Lane 2: GAD-His tag Transfected 293T cell lysate;;)
Mouse His tag Monoclonal Antibody
His tag
Applications
ELISA, Western Blot
Purity
By protein-A affinity chromatography
Synonyms
His tag; Monoclonal Antibody; 6 His epitope tag antibody; Hexa His tag antibody; HHHHHH epitope tag antibody; HHHHHH tag antibody; His tag antibody; Polyhistidine Tag antibody; anti-His tag antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1 kappa
Clone Number
AT1D6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In Phosphate-Buffered Saline (pH 7.4) with 0.02% Sodium Azide, 10% Glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-His tag antibody
ELISA (EIA), Western Blot (WB)
Application Notes
The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Immunogen
6x His synthetic peptide
Conjugation
Unconjugated
Preparation and Storage
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human His tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1: OTUB2-His tag Transfected 293T cell lysate;;Lane 2: GAD-His tag Transfected 293T cell lysate;;)
Western Blot (WB)
(The cell lysates(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human His tag antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.;;Lane 1: OTUB2-His tag Transfected 293T cell lysate;;Lane 2: GAD-His tag Transfected 293T cell lysate;;)
Related Product Information for anti-His tag antibody
A polyhistidine-tag is an amino acid motif in proteins that consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, His6 tag and by the trademarked name His-tag.
Product Categories/Family for anti-His tag antibody
References
O Hare, Simura, Gibson; et al. (2006). Biomolecular Methods. 38 (22): 873-885.;;Li Y et al. from the Mariana Trench. Mar Drugs 17:N/A (2019).;;Andersen, Kasper R.; Leksa, Nina C.; Schwartz, Thomas U. (2013).;;
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Product Notes
The His tag (Catalog #AAA200507) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's His tag can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the His tag for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "His tag, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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