Typical Testing Data/Standard Curve (for reference only)
Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein ELISA Kit | PREX2 elisa kit
Human Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein ELISA Kit
Gene Names
PREX2; DEP.2; DEPDC2; P-REX2; PPP1R129; 6230420N16Rik
Reactivity
Human
Synonyms
Phosphatidylinositol 3; 4; 5-trisphosphate-dependent Rac exchanger 2 protein; Human Phosphatidylinositol 3; 5-trisphosphate-dependent Rac exchanger 2 protein ELISA Kit; P-Rex2; PtdIns(3; 5)-dependent Rac exchanger 2; DEP domain-containing protein 2; PREX2; DEPDC2; PREX2 elisa kit
Reactivity
Human
Specificity
Recombinant and natural Human P-Rex2
Sequence Length
1606
Samples
Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
Detection Range
78-5000 pg/mL
Sensitivity
<34 pg/mL
Intra-assay Precision
Intra-Assay CV: <=3.9%
Inter-assay Precision
Inter-Assay CV: <=7.2%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PREX2 elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
112,404 Da
NCBI Official Full Name
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein isoform a
NCBI Official Synonym Full Names
phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 2
NCBI Official Symbol
PREX2
NCBI Official Synonym Symbols
DEP.2; DEPDC2; P-REX2; PPP1R129; 6230420N16Rik
NCBI Protein Information
phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
UniProt Protein Name
Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein
UniProt Gene Name
PREX2
UniProt Synonym Gene Names
DEPDC2; P-Rex2; PtdIns(3,4,5)-dependent Rac exchanger 2
UniProt Entry Name
PREX2_HUMAN
Uniprot Description
PREX2: Functions as a RAC1 guanine nucleotide exchange factor (GEF), activating Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. Mediates the activation of RAC1 in a PI3K-dependent manner. May be an important mediator of Rac signaling, acting directly downstream of both G protein- coupled receptors and phosphoinositide 3-kinase. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: GEFs; Phosphatase, regulatory subunit; GEFs, Rac/Rho
Chromosomal Location of Human Ortholog: 8q13.2
Cellular Component: intracellular; plasma membrane
Molecular Function: GTPase activator activity; Rac guanyl-nucleotide exchange factor activity
Biological Process: adult locomotory behavior; dendrite morphogenesis; G-protein coupled receptor protein signaling pathway; phosphoinositide 3-kinase cascade; positive regulation of GTPase activity; regulation of Rho protein signal transduction
Research Articles on PREX2
Similar Products
Product Notes
The Human PREX2 prex2 (Catalog #AAA2881972) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2881972 ELISA Kit recognizes Human PREX2. It is sometimes possible for the material contained within the vial of "Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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