Nuclear RNA Export Factor 1 Recombinant Protein | NXF1 recombinant protein

Recombinant Nuclear RNA Export Factor 1 (NXF1)

Cat. Num. AAA2033024

• Gene Names: NXF1; TAP; MEX67
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Nuclear RNA Export Factor 1; Recombinant Nuclear RNA Export Factor 1 (NXF1); TAP; MEX67; Tip Associating Protein; Tip-associated protein; mRNA export factor TAP; NXF1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Sequence: ADEGKSYNE HDDRVSFPQR RKKGRGPFRW KCGVGNRRSG RGGSGIRSSR FEEDDGDVAM NDPQDGPRVR FNPYTTRPNR RRDTWHDRDR IHVTVRRDRA PQERGGAGTS QDGTTKNWFK ITIPYGKKYD KMWLLSMIQS KCSVPFNPIE FHYENTRAHFFVENATTASA LKAVNYKIQD RENGRISIII NSSAPPY
• Sequence Length: 619

• Applicable Applications for NXF1 recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 26.3kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Ala2~Tyr197
• Organism Species: Rat
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4220554
• NCBI GeneID: 10482
• UniProt Accession #: Q9UBU9; Q99799; Q9UQL2; B4E269
• Molecular Weight: 40,476 Da
• NCBI Official Full Name: nuclear RNA export factor 1
• NCBI Official Synonym Full Names: nuclear RNA export factor 1
• NCBI Official Symbol: NXF1
• NCBI Official Synonym Symbols: TAP; MEX67
• NCBI Protein Information: nuclear RNA export factor 1
• UniProt Protein Name: Nuclear RNA export factor 1
• Protein Family: Nuclear RNA export factor
• UniProt Gene Name: NXF1
• UniProt Synonym Gene Names: TAP
• UniProt Entry Name: NXF1_HUMAN

NCBI Description

This gene is one member of a family of nuclear RNA export factor genes. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1 (NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. Alternative splicing seems to be a common mechanism in this gene family. The encoded protein of this gene shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. The encoded protein overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the nuclear export of mRNA species bearing retroviral constitutive transport elements (CTE) and in the export of mRNA from the nucleus to the cytoplasm (TAP/NFX1 pathway). The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with ALYREF/THOC4 and THOC5 components of the TREX complex. ALYREF/THOC4-bound mRNA is thought to be transferred to the NXF1-NXT1 heterodimer for export.