Western Blot (WB)
(Livin/ML-IAP mAb tested by Western blot. Livin detection by Western blot. The detection of Livin was performed using extract from a cell that had been transfected with human Livin cDNA. A protein of 31 kDa was detected using Livin/ML-IAP mAb at a 2 ug/ml dilution.)
Rabbit anti-Human Livin/ML-IAP Monoclonal Antibody | anti-Livin/ML-IAP antibody
Livin/ML-IAP antibody (mAb)(Clone 88C570)
Gene Names
BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Affinity Purified
Synonyms
Livin/ML-IAP; Monoclonal Antibody; Livin/ML-IAP antibody (mAb)(Clone 88C570); BIRC7; KIAP; MLIAP; RNF50; anti-Livin/ML-IAP antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
88C570
Purity/Purification
Affinity Purified
Form/Format
PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Concentration
0.5ug/ul (varies by lot)
Applicable Applications for anti-Livin/ML-IAP antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Applications Validated:
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
WB: 1-2ug/ml dilution
For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background.
Immunogen
This Livin/ML-IAP antibody was raised against a GST-fusion protein corresponding to human Livin/ML-IAP.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Livin/ML-IAP mAb tested by Western blot. Livin detection by Western blot. The detection of Livin was performed using extract from a cell that had been transfected with human Livin cDNA. A protein of 31 kDa was detected using Livin/ML-IAP mAb at a 2 ug/ml dilution.)
Western Blot (WB)
(Livin/ML-IAP mAb tested by Western blot. Livin detection by Western blot. The detection of Livin was performed using extract from a cell that had been transfected with human Livin cDNA. A protein of 31 kDa was detected using Livin/ML-IAP mAb at a 2 ug/ml dilution.)
Related Product Information for anti-Livin/ML-IAP antibody
Short Description: Our Livin/ML-IAP antibody (mAb)(Clone 88C570) was raised in a Mouse host. It has been validated for use in Immunohistochemistry and Western blot; it has been shown to react with Human samples.
Background: Livin (ML-IAP or BIRC7) protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, Livin (ML-IAP or BIRC7) inhibits Caspase-3 and proteolytic activation of pro-Caspase-9. Isoform 1 of Livin (ML-IAP or BIRC7) blocks staurosporine-induced apoptosis, while isoform 2 blocks etoposide-induced apoptosis.
Background: Livin (ML-IAP or BIRC7) protects against apoptosis induced by TNF or by chemical agents such as adriamycin, etoposide or staurosporine. Suppression of apoptosis is mediated by activation of MAPK8/JNK1, and possibly also MAPK9/JNK2. This activation depends on TAB1 and NR2C2/TAK1. In vitro, Livin (ML-IAP or BIRC7) inhibits Caspase-3 and proteolytic activation of pro-Caspase-9. Isoform 1 of Livin (ML-IAP or BIRC7) blocks staurosporine-induced apoptosis, while isoform 2 blocks etoposide-induced apoptosis.
Product Categories/Family for anti-Livin/ML-IAP antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34 kDa (318 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
baculoviral IAP repeat-containing protein 7 isoform alpha
NCBI Official Synonym Full Names
baculoviral IAP repeat containing 7
NCBI Official Symbol
BIRC7
NCBI Official Synonym Symbols
KIAP; LIVIN; MLIAP; RNF50; ML-IAP
NCBI Protein Information
baculoviral IAP repeat-containing protein 7
UniProt Protein Name
Baculoviral IAP repeat-containing protein 7
UniProt Gene Name
BIRC7
UniProt Synonym Gene Names
KIAP; LIVIN; MLIAP; RNF50; KIAP; ML-IAP; Truncated livin; p30-Livin; tLivin
UniProt Entry Name
BIRC7_HUMAN
NCBI Description
This gene encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jul 2013]
Research Articles on Livin/ML-IAP
Similar Products
Product Notes
The Livin/ML-IAP birc7 (Catalog #AAA388699) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Livin/ML-IAP antibody (mAb)(Clone 88C570) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Livin/ML-IAP can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). Applications Validated: WB: 1-2ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Researchers should empirically determine the suitability of the Livin/ML-IAP birc7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Livin/ML-IAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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