ELISA (EIA)
Rabbit anti-Mouse TIGIT, IgG, kappa Monoclonal Antibody | anti-TIGIT antibody
Anti-TIGIT, Rabbit IgG, kappa Antibody
Gene Names
Tigit; Vstm3
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A purification
Synonyms
TIGIT; IgG; kappa; Monoclonal Antibody; Anti-TIGIT; Rabbit IgG; kappa Antibody; T Cell Immunoreceptor With Ig And ITIM Domains; V-Set And Immunoglobulin Domain Containing Protein 9; V-Set And Transmembrane Domain Containing Protein 3; VSIG9; VSTM3; Washington University Cell Adhesion Molecule; WUCAM; anti-TIGIT antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
4D4
Specificity
Recombinant monoclonal antibody manufactured using Recombinant Platform with variable regions from the hybridoma 4D4 to detect TIGIT expression in mouse. 4D4 is an agonistic anti-TIGIT antibody, and addition of anti-CD3/anti-CD28 stimulated T cells to plate bound 4D4 is known to inhibit their proliferation. Further, 4D4 has been used in order to identify genes that are downstream of TIGIT signaling in Tregs, as TIGIT engagement might modulate the Treg phenotype in tumor tissue.
Purity/Purification
Protein A purification
Form/Format
Colorless liquid. In PBS with 0.02% Proclin 300
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TIGIT antibody
Agonist, Flow Cytometry (FC/FACS)
Antibody Type
Recombinant Antibody
Immunogen
Armenian hamsters were immunized with recombinant mouse TIGIT tetramers by a combination of s.c. and food pad immunization and booster injections. Draining lymph nodes were fused with Sp2/0-Ag14 cells, selected in HAT medium and supernatants were screened for specificity by ELISA and FC using TIGIT-transfectants
Preparation and Storage
Store at -20 degree C
The antibody solution should be gently mixed before use.
Shelf life: ~12 months
The antibody solution should be gently mixed before use.
Shelf life: ~12 months
ELISA (EIA)
ELISA (EIA)
Related Product Information for anti-TIGIT antibody
Recombinant monoclonal antibody to TIGIT. T cell immunoreceptor with Ig and ITIM domains (TIGIT), also known as VSTM3, is a type I transmembrane protein, which belongs to the PVR family of immunoglobulin-like domain containing proteins. It is composed of an immunoglobulin variable domain, a transmembrane domain and an immunoreceptor tyrosine-based inhibitory motif (ITIM). TIGIT is expressed on activated T cells, follicular T helper, memory T cells, and regulatory T cells and NK cells. TIGIT binds to CD155 (PVR) with high affinity and it regulates T-cell mediated immunity via the CD226/TIGIT-PVR pathway. TIGIT also serves as a negative regulator of NK and T cell activation.
Product Categories/Family for anti-TIGIT antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
26,959 Da
NCBI Official Full Name
T-cell immunoreceptor with Ig and ITIM domains
NCBI Official Synonym Full Names
T cell immunoreceptor with Ig and ITIM domains
NCBI Official Symbol
Tigit
NCBI Official Synonym Symbols
Vstm3
NCBI Protein Information
T-cell immunoreceptor with Ig and ITIM domains
UniProt Protein Name
T-cell immunoreceptor with Ig and ITIM domains
Protein Family
UniProt Gene Name
Tigit
Uniprot Description
Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Research Articles on TIGIT
Similar Products
Product Notes
The TIGIT tigit (Catalog #AAA847835) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-TIGIT, Rabbit IgG, kappa Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TIGIT, IgG, kappa can be used in a range of immunoassay formats including, but not limited to, Agonist, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the TIGIT tigit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIGIT, IgG, kappa, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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