Rabbit anti-Mouse PSGL-1/CD162 Monoclonal Antibody | anti-SELPLG antibody

Recombinant Anti-PSGL-1/CD162 Antibody (FITC), Rabbit Monoclonal

Cat. Num. AAA8112054

• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: PSGL-1/CD162; Monoclonal Antibody; Recombinant Anti-PSGL-1/CD162 Antibody (FITC); Rabbit Monoclonal; PSGL-1/CD162 Antibody (FITC); Rabbit MAb; selectin P ligand; Anti-CD162 Antibody; Anti-Psgl-1 Antibody; Anti-Psgl1 Antibody; Anti-Selp1 Antibody; Anti-Selpl Antibody; anti-SELPLG antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 001
• Specificity: Mouse PSGL-1/CD162
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-SELPLG antibody: Flow Cytometry (FC/FACS/FCM)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Mouse PSGL-1/CD162 Protein
• Conjugation: FITC
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse PSGL-1/CD162 (rM PSGL-1/CD162) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Mouse SELPLG(CD162) expression on BABL/c splenocytes. Cells were stained with FITC-conjugated anti-Mouse SELPLG(CD162). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-SELPLG antibody

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

References

1. Sako, D. et al., 1993, Cell. 75: 1179-1186.
2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.
3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.
4. Vandendries, E.R.et al., 2004, Thromb. Haemost. 92: 459-466..
5. Pouyani, T. et al., 1995, Cell. 83: 333-343.

NCBI and Uniprot Product Information

• UniProt Accession #: Q62170
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• Protein Family: P-selectin glycoprotein ligand
• UniProt Gene Name: Selplg
• UniProt Synonym Gene Names: Selp1; Selpl; PSGL-1

Uniprot Description

A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.