PSGL-1/CD162 Recombinant Protein | SELPLG recombinant protein

PSGL-1/CD162 Protein, Mouse, Recombinant (hFc Tag)

Cat. Num. AAA8122977

• Purity: >85% as determined by SDS-PAGE
• Synonyms: PSGL-1/CD162; PSGL-1/CD162 Protein; Mouse; Recombinant (hFc Tag); Mouse PSGL-1/CD162/SELPLG Protein (Fc Tag); CD162 Protein; Psgl-1 Protein; Psgl1 Protein; Selp1 Protein; Selpl Protein; selectin P ligand; SELPLG recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Cys307

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 18
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse SELPLG (Q62170)(Met 1-Cys 307) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SELPLG recombinant protein

Background: P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca²⁺-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

References

1. Sako, D. et al., 1993, Cell. 75: 1179-1186.
2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.
3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.
4. Vandendries, E.R.et al., 2004, Thromb. Haemost. 92: 459-466..
5. Pouyani, T. et al., 1995, Cell. 83: 333-343.

NCBI and Uniprot Product Information

• UniProt Accession #: Q62170
• Molecular Weight: The secreted recombinant mouse SELPLG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 531 amino acids and has a calculated molecular mass of 57.4kDa. As a result of glycosylation, the apparent molecular mass of rmSELPLG/Fc monomer is app
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• Protein Family: P-selectin glycoprotein ligand
• UniProt Gene Name: Selplg
• UniProt Synonym Gene Names: Selp1; Selpl; PSGL-1

Uniprot Description

A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E- and P-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture.