Rabbit anti-Human EAPII Polyclonal Antibody | anti-EAPII antibody

EAPII, CT (TDP2, EAP2, TTRAP, Tyrosyl-DNA phosphodiesterase 2, 5'-tyrosyl-DNA phosphodiesterase, ETS1-associated protein 2, ETS1-associated protein II, TRAF and TNF receptor-associated protein) (Biotin)

Cat. Num. AAA6294139

• Gene Names: TDP2; EAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EAPII; Polyclonal Antibody; CT (TDP2; EAP2; TTRAP; Tyrosyl-DNA phosphodiesterase 2; 5'-tyrosyl-DNA phosphodiesterase; ETS1-associated protein 2; ETS1-associated protein II; TRAF and TNF receptor-associated protein) (Biotin); TDP2; TRAF and TNF receptor-associated protein; anti-EAPII antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-EAPII antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IF: 1:10-50; IHC: 1:10-50; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: EAPII antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 253-281 amino acids from the C-terminal region of human EAPII.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(EAPII Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the EAPII antibody detected the EAPII protein (arrow).)

Immunohistochemistry (IHC)

(EAPII Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of EAPII Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of EAPII Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(EAPII Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EAPII antibody

This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors.

Product Categories/Family for anti-EAPII antibody

Antibodies; Enzymes; Phosphodiesterase

References

Shimada, M., et al. Hum. Genet. 128(4):433-441(2010). Wang, B.Y., et al. Mol. Biol. Rep. 37(6):2809-2816(2010). Iijima, M., et al. Neurology 73(17):1348-1352(2009). Cortes Ledesma, F., et al. Nature 461(7264):674-678(2009). Zhang, J.Q., et al. Biochem. Biophys. Res. Commun. 387(2):256-260(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 23510348
• NCBI GeneID: 51567
• NCBI Accession #: NP_057698
• NCBI GenBank Nucleotide #: NM_016614.2
• UniProt Accession #: O95551; Q2TBE2; Q5JYM0; Q7Z6U5; Q9NUK5; Q9NYY9; B4DKL8; B4DQ95
• Molecular Weight: 43.3 kDa (385aa) confirmed by MALDI-TOF
• NCBI Official Full Name: tyrosyl-DNA phosphodiesterase 2
• NCBI Official Synonym Full Names: tyrosyl-DNA phosphodiesterase 2
• NCBI Official Symbol: TDP2
• NCBI Official Synonym Symbols: EAP2; AD022; EAPII; TTRAP; hTDP2; dJ30M3.3
• NCBI Protein Information: tyrosyl-DNA phosphodiesterase 2
• UniProt Protein Name: Tyrosyl-DNA phosphodiesterase 2
• UniProt Gene Name: TDP2
• UniProt Synonym Gene Names: EAP2; TTRAP; Tyr-DNA phosphodiesterase 2; hTDP2; 5'-Tyr-DNA phosphodiesterase; EAPII
• UniProt Entry Name: TYDP2_HUMAN

NCBI Description

This gene encodes a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. [provided by RefSeq, Jul 2008]

Uniprot Description

TTRAP: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has preference for single-stranded DNA or duplex DNA with a 4 base pair overhang as substrate. Has also 3'- tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. Constitutes the major if not only 5'- tyrosyl-DNA phosphodiesterase in cells. Also acts as a 5'-tyrosyl- RNA phosphodiesterase following picornavirus infection: its activity is hijacked by picornavirus and acts by specifically cleaving the protein-RNA covalent linkage generated during the viral genomic RNA replication steps of a picornavirus infection, without impairing the integrity of viral RNA. Also acts as an adapter by participating to the specific activation of MAP3K7/TAK1 in response to TGF-beta: associates with components of the TGF- beta receptor-TRAF6-TAK1 signaling module and promotes their ubiquitination dependent complex formation. Involved in non- canonical TGF-beta induced signaling routes. May also act as a negative regulator of ETS1 and may inhibit NF-kappa-B activation. Acts as a regulator of ribosome biogenesis following stress. Belongs to the CCR4/nocturin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Inhibitor; EC 3.1.4.-

Chromosomal Location of Human Ortholog: 6p22.3-p22.1

Cellular Component: nucleoplasm; PML body; intercellular bridge; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; nuclease activity; manganese ion binding; magnesium ion binding; transcription corepressor activity; single-stranded DNA binding

Biological Process: cell surface receptor linked signal transduction; double-strand break repair