PSGL-1 / CD162 / SELPLG recombinant protein

Recombinant Mouse PSGL-1 / CD162 / SELPLG Protein (Fc tag)

Cat. Num. AAA2546795

• Gene Names: Selplg; CD162; Psgl1; Selp1; Selpl; Psgl-1
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: PSGL-1 / CD162 / SELPLG; Recombinant Mouse PSGL-1 / CD162 / SELPLG Protein (Fc tag); CD162; Psgl-1; Psgl1; Selp1; Selpl; PSGL-1 / CD162 / SELPLG recombinant protein

• Host: Human Cells.
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween 80
• Sequence Length: 397

• Application Notes: The secreted recombinant mouse SELPLG/Fc is a disulfide-linked homodimer. The reduced monomer comprises 531 amino acids and has a calculated molecular mass of 57.4 kDa. As a result of glycosylation, the apparent molecular mass of rmSELPLG/Fc monomer is approximately 82 kDa in SDS-PAGE under reducing conditions.
• Source: Mouse
• Predicted N Terminal: Leu 18
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse SELPLG (Q62170) (Met 1-Cys 307) was fused with the Fc region of human IgG1 at the C-terminus.
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Molecular Mass: The secreted recombinant mouse SELPLG /Fc is a disulfide-linked homodimer. The reduced monomer comprises 531 amino acids and has a calculated molecular mass of 57.4 kDa. As a result of glycosylation, the apparent molecular mass of rmSELPLG/Fc monmer is approximately 82..
• Reconstitution: It is recommended that sterile water(200uL) be addded to the vial to prepare a stock solution of 0.25 mg/mL. ; ; Concentration is measured by UV- Vis.
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C . It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for PSGL-1 / CD162 / SELPLG recombinant protein

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinitycounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~120 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

Description: A DNA sequence encoding the extracellular domain of mouse SELPLG (Q62170) (Met 1-Cys 307) was fused with the Fc region of human IgG1 at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 341942014
• NCBI GeneID: 20345
• UniProt Accession #: Q62170
• NCBI Official Full Name: P-selectin glycoprotein ligand 1
• NCBI Official Synonym Full Names: selectin, platelet (p-selectin) ligand
• NCBI Official Symbol: Selplg
• NCBI Official Synonym Symbols: CD162; Psgl1; Selp1; Selpl; Psgl-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• UniProt Gene Name: Selplg
• UniProt Synonym Gene Names: Selp1; Selpl; PSGL-1

Uniprot Description

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5 F|5 55.59 cM

Cellular Component: plasma membrane; uropod

Molecular Function: protein binding

Biological Process: leukocyte adhesive activation; leukocyte tethering or rolling