Mouse anti-Human PSGL-1/CD162 Monoclonal Antibody | anti-SELPLG antibody

Anti-PSGL-1/CD162 Antibody (APC), Mouse Monoclonal

Cat. Num. AAA8107119

• Gene Names: SELPLG; CLA; CD162; PSGL1; PSGL-1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Protein A
• Synonyms: PSGL-1/CD162; Monoclonal Antibody; Anti-PSGL-1/CD162 Antibody (APC); Mouse Monoclonal; PSGL-1/CD162 Antibody (APC); Mouse MAb; selectin P ligand; Anti-CD162 Antibody; Anti-CLA Antibody; Anti-PSGL-1 Antibody; Anti-PSGL1 Antibody; anti-SELPLG antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 07
• Specificity: Human PSGL-1/CD162
• Purity/Purification: Protein A
• Form/Format: Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Concentration: 10ul/Test, 0.1mg/ml (varies by lot)

• Applicable Applications for anti-SELPLG antibody: Flow Cytometry (FC/FACS/FCM)
• Immunogen: Recombinant Human PSGL-1/CD162 Protein
• Conjugation: APC
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human PSGL-1/CD162 (rh PSGL-1/CD162) and conjugated with APC under optimum conditions, the unreacted APC was removed.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Human SELPLG(CD162) expression on human whole blood lymphocytes. Cells were stained with APC-conjugated anti-Human SELPLG(CD162). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

Related Product Information for anti-SELPLG antibody

P-selectin glycoprotein ligand-1 (PSGL-1), also known as SELPLG or CD162, is the high affinity ounter-receptor for P-selectin on expressed on activated endothelial cells and platelets. PSGL-1 is a mucin-type glycoprotein, expressed on leukocytes and platelets as a homodimer of two disulfide-linked subunits of ~12 kD. As cell adhesion molecules, multiple studies have shown that PSGL-1/ P-selectin interaction is required for the normal recruitment of leukocytes during inflammatory reactions, and also participates in hemostatic responses. PSGL-1 protein requires two distinct posttranslational modifications for the Ca2+-dependent recognition by the lectin domain of P-selectin, that is tyrosine sulfation and specific O-linked glycosylation (sialic acid and fucose). PSGL-1 can also bind to other two members of the selectin family, E-selectin (endothelial) and L-selectin (leukocyte), but binds best to P-selectin.

References

1. Sako, D. et al., 1993, Cell. 75: 1179-1186.
2. Wilkins, P. P. et al., 1995, J. Biol. Chem. 270: 22677-22680.
3. Frenette, P. S. et al., 2000, J. Exp. Med. 191: 1413-1422.
4. Vandendries, E.R.et al., 2004, Thromb. Haemost. 92: 459-466..
5. Pouyani, T. et al., 1995, Cell. 83: 333-343.

NCBI and Uniprot Product Information

• NCBI GI #: 331284238
• NCBI GeneID: 6404
• NCBI Accession #: NP_001193538.1
• NCBI GenBank Nucleotide #: NM_001206609.1
• UniProt Accession #: Q14242; Q12775; Q6GTW7; Q8N7J7; A8K2Y0; B4DQC3; B7Z5C7; J3KMX6
• Molecular Weight: 43,201 Da
• NCBI Official Full Name: P-selectin glycoprotein ligand 1 isoform 1
• NCBI Official Synonym Full Names: selectin P ligand
• NCBI Official Symbol: SELPLG
• NCBI Official Synonym Symbols: CLA; CD162; PSGL1; PSGL-1
• NCBI Protein Information: P-selectin glycoprotein ligand 1; cutaneous lymphocyte-associated associated antigen
• UniProt Protein Name: P-selectin glycoprotein ligand 1
• Protein Family: P-selectin glycoprotein ligand
• UniProt Gene Name: SELPLG
• UniProt Synonym Gene Names: PSGL-1
• UniProt Entry Name: SELPL_HUMAN

NCBI Description

This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]

Uniprot Description

SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: protein binding; receptor binding

Biological Process: blood coagulation; cell adhesion; leukocyte tethering or rolling; leukocyte adhesive activation; leukocyte migration