Flow Cytometry (FC/FACS)
Mouse anti-Human, Rat Peroxiredoxin 6 Monoclonal Antibody | anti-PRDX6 antibody
Anti-Peroxiredoxin 6 Antibody (Monoclonal, 6I8)
Gene Names
PRDX6; PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Immunogen affinity purified.
Synonyms
Peroxiredoxin 6; Monoclonal Antibody; Anti-Peroxiredoxin 6 Antibody (Monoclonal; 6I8); 1 Cys antibody; 1 Cys peroxiredoxin antibody; 1 Cys PRX antibody; 1 cysPrx antibody; 1-Cys peroxiredoxin antibody; 1-Cys PRX antibody; 24 kDa protein antibody; 9430088D19Rik antibody; AA690119 antibody; Acidic calcium independent phospholipase A2 antibody; Acidic calcium-independent phospholipase A2 antibody; aiPLA2 antibody; Antioxidant protein 2 antibody; AOP2 antibody; Aop2 rs3 antibody; Brp 12 antibody; Ciliary body glutathione peroxidase antibody; CP 3 antibody; EC 1.11.1.15 antibody; EC 1.11.1.7 antibody; EC 3.1.1. antibody; GPx antibody; KIAA0106 antibody; Liver 2D page spot 40 antibody; Ltw4 antibody; Lvtw 4 antibody; MGC46173 antibody; mKIAA0106 antibody; Non selenium glutathione peroxidase antibody; Non-selenium glutathione peroxidase antibody; NSGPx antibody; ORF06 antibody; OTTHUMP00000032693 antibody; p29 antibody; Peroxiredoxin-6 antibody; Peroxiredoxin6 antibody; PHGPx antibody; Phospholipase A2 lysosomal antibody; PLA2 antibody; PRDX 6 antibody; Prdx5 antibody; PRDX6 antibody; Prdx6 rs3 antibody; PRDX6_HUMAN antibody; PRX antibody; Red blood cells page spot 12 antibody; Thiol specific antioxidant protein antibody; anti-PRDX6 antibody
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
6I8
Specificity
No cross reactivity with other proteins.
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
224
Applicable Applications for anti-PRDX6 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
WB: 0.25-0.5ug/ml (Human;Rat)
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Tested Species: In-house tested species with positive results.
IHC-P (Embedded Section): 0.5-1ug/ml (Human)
ICC: 2ug/ml (Human)
IF: 2ug/ml (Human)
FC/FACS: 1-3ug/1x106 cells (Human)
Tested Species: In-house tested species with positive results.
Immunogen
E Coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224). Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6, respectively.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
Store at -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen store at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Immunofluorescence (IF)
Immunofluorescence (IF)
Western Blot (WB)
Western Blot (WB)
Related Product Information for anti-PRDX6 antibody
Description: Mouse IgG monoclonal antibody for peroxiredoxin 6 detection. Tested with WB, IHC-P, ICC/IF, FCM in Human;Rat.
Background: PRDX6 is also known as PRX, p29 or AOP2. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
Background: PRDX6 is also known as PRX, p29 or AOP2. The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury.
References
1. Ambruso DR, et al. Peroxiredoxin 6 translocates to the plasma membrane during neutrophil activation and is required for optimal NADPH oxidase activity. Biochim Biophys Acta, 2012 Feb.
2. Chhunchha B, et al. Aberrant sumoylation signaling evoked by reactive oxygen species impairs protective function of Prdx6 by destabilization and repression of its transcription. FEBS J, 2014 Aug.
3. Guo HW, et al. Expression of peroxiredoxin 6 in gastric cancer and its clinical significance. Zhonghua Yi Xue Za Zhi, 2012 Sep 11.
2. Chhunchha B, et al. Aberrant sumoylation signaling evoked by reactive oxygen species impairs protective function of Prdx6 by destabilization and repression of its transcription. FEBS J, 2014 Aug.
3. Guo HW, et al. Expression of peroxiredoxin 6 in gastric cancer and its clinical significance. Zhonghua Yi Xue Za Zhi, 2012 Sep 11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
NCBI Official Full Name
Peroxiredoxin-6
NCBI Official Synonym Full Names
peroxiredoxin 6
NCBI Official Symbol
PRDX6
NCBI Official Synonym Symbols
PRX; p29; AOP2; 1-Cys; NSGPx; aiPLA2; HEL-S-128m
NCBI Protein Information
peroxiredoxin-6
UniProt Protein Name
Peroxiredoxin-6
Protein Family
UniProt Gene Name
PRDX6
UniProt Synonym Gene Names
AOP2; KIAA0106; 1-Cys PRX; aiPLA2; NSGPx
UniProt Entry Name
PRDX6_HUMAN
NCBI Description
The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]
Uniprot Description
PRDX6: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Belongs to the AhpC/TSA family. Rehydrin subfamily.
Protein type: Amino Acid Metabolism - phenylalanine; Oxidoreductase; Energy Metabolism - methane; EC 1.11.1.9; EC 1.11.1.15; Phospholipase
Chromosomal Location of Human Ortholog: 1q25.1
Cellular Component: extracellular space; membrane; lysosome; cytoplasmic membrane-bound vesicle; cytoplasm; cytosol
Molecular Function: antioxidant activity; phospholipase A2 activity; protein binding; peroxiredoxin activity; glutathione peroxidase activity
Biological Process: response to reactive oxygen species; phospholipid catabolic process; hydrogen peroxide catabolic process; response to oxidative stress
Research Articles on PRDX6
Similar Products
Product Notes
The PRDX6 prdx6 (Catalog #AAA1753231) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Anti-Peroxiredoxin 6 Antibody (Monoclonal, 6I8) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxiredoxin 6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). WB: 0.25-0.5ug/ml (Human;Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Human) ICC: 2ug/ml (Human) IF: 2ug/ml (Human) FC/FACS: 1-3ug/1x106 cells (Human) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the PRDX6 prdx6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxiredoxin 6, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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