Immunoprecipitation (IP)
(PRDX6 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PRDX6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRDX6 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)
Rabbit anti-Human Peroxiredoxin 6 Polyclonal Antibody | anti-PRDX6 antibody
Anti-Peroxiredoxin 6 Antibody, Rabbit Polyclonal
IP: 1-4uL/mg of lysate
Immunoprecipitation (IP)
(PRDX6 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PRDX6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRDX6 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)
Immunoprecipitation (IP)
(PRDX6 was immunoprecipitated using:Lane A:0.5 mg HepG2 Whole Cell LysateLane B:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-PRDX6 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-PRDX6 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)
Western Blot (WB)
(Anti-PRDX6 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: MCF7 Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa)
Western Blot (WB)
(Anti-PRDX6 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: Hela Whole Cell LysateLane C: MCF7 Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa)
NCBI and Uniprot Product Information
Calculated: 25kDa
NCBI Description
The protein encoded by this gene is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. [provided by RefSeq, Jul 2008]
Uniprot Description
PRDX6: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Belongs to the AhpC/TSA family. Rehydrin subfamily.
Protein type: Amino Acid Metabolism - phenylalanine; Oxidoreductase; Energy Metabolism - methane; EC 1.11.1.9; EC 1.11.1.15; Phospholipase
Chromosomal Location of Human Ortholog: 1q25.1
Cellular Component: extracellular space; membrane; lysosome; cytoplasmic membrane-bound vesicle; cytoplasm; cytosol
Molecular Function: antioxidant activity; phospholipase A2 activity; protein binding; peroxiredoxin activity; glutathione peroxidase activity
Biological Process: response to reactive oxygen species; phospholipid catabolic process; hydrogen peroxide catabolic process; response to oxidative stress
Research Articles on PRDX6
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Product Notes
The PRDX6 prdx6 (Catalog #AAA8105170) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Peroxiredoxin 6 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Peroxiredoxin 6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the PRDX6 prdx6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Peroxiredoxin 6, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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