Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit | PRDX6 elisa kit

Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit

Cat. Num. AAA9717300

• Gene Names: Prdx6; GPx; Aop2; CP-3; Ltw4; Ltw-4; NSGPx; ORF06; Prdx5; Brp-12; Lvtw-4; aiPLA2; 1-cysPrx; AA690119; 1-Cys Prx; 9430088D19Rik
• Reactivity: Mouse
• Synonyms: Peroxiredoxin-6 (PRDX6); Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit; PRDX6; 1-Cys; AOP2; KIAA0106; MGC46173; NSGPx; PRX; aiPLA2; p29; 1-Cys peroxiredoxin; acidic calcium-independent phospholipase A2; antioxidant protein 2; non-selenium glutathione peroxidase; PRDX6 elisa kit

• Reactivity: Mouse
• Specificity: Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit has high sensitivity and excellent specificity for detection of Mouse PRDX6. No significant cross-reactivity or interference between Mouse PRDX6 and analogues was observed.
• Sequence Length: 224

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Mouse Peroxiredoxin-6 (PRDX6) Standard Curve.)

Related Product Information for PRDX6 elisa kit

Description: This Mouse Peroxiredoxin-6 (PRDX6) ELISA Kit employs a two-site sandwich ELISA to quantitate PRDX6 in samples. An antibody specific for PRDX6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRDX6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRDX6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRDX6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Peroxiredoxin-6 is a member of the thiol-specific antioxidant protein family. This protein is a bifunctional enzyme with two distinct active sites. It is involved in redox regulation of the cell; it can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. It may play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Kim et al. (1997) isolated a calcium-independent lysosomal PLA2 from a human myeloblast cell line. The predicted 224-amino acid protein contained the PLA2 catalytic motif, but no other significant homology to known phospholipases. Expressed protein had significant PLA2 activity on several substrates.

NCBI and Uniprot Product Information

• NCBI GI #: 6671549
• NCBI GeneID: 11758
• NCBI Accession #: NP_031479.1
• NCBI GenBank Nucleotide #: NP_031479.1
• UniProt Accession #: O08709
• NCBI Official Full Name: peroxiredoxin-6 isoform 1
• NCBI Official Synonym Full Names: peroxiredoxin 6
• NCBI Official Symbol: Prdx6
• NCBI Official Synonym Symbols: GPx; Aop2; CP-3; Ltw4; Ltw-4; NSGPx; ORF06; Prdx5; Brp-12; Lvtw-4; aiPLA2; 1-cysPrx; AA690119; 1-Cys Prx; 9430088D19Rik
• NCBI Protein Information: peroxiredoxin-6
• UniProt Protein Name: Peroxiredoxin-6
• Protein Family: Peroxiredoxin
• UniProt Gene Name: Prdx6
• UniProt Synonym Gene Names: Aop2; Ltw4; Prdx5; 1-Cys PRX; aiPLA2; NSGPx

NCBI Description

This gene encodes a member of the peroxiredoxin family of peroxidases. The encoded protein is a bifunctional enzyme that has glutathione peroxidase and phospholipase activities. This protein is an antioxidant that reduces peroxidized membrane phospholipids and plays an important role in phospholipid homeostasis based on its ability to generate lysophospholipid substrate for the remodeling pathway of phospholipid synthesis. Mice lacking this gene are sensitive to oxidant stress, have altered lung phospholipid metabolism and susceptible to skin tumorigenesis. Alternate splicing of this gene results in multiple transcript variants. A pseudogene of this gene is found on chromosome 4. [provided by RefSeq, Dec 2014]

Uniprot Description

Involved in redox regulation of the cell. Can reduce H2O2 and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury ().