Rabbit PDK-1 Monoclonal Antibody | anti-pdk-1 antibody

PDK-1 (Pyruvate Dehydrogenase Kinase-1, PDHK1)

Cat. Num. AAA606108

• Reactivity: Human, Monkey, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation
• Synonyms: PDK-1; Monoclonal Antibody; PDK-1 (Pyruvate Dehydrogenase Kinase-1; PDHK1); Anti -PDK-1 (Pyruvate Dehydrogenase Kinase-1; anti-pdk-1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 10E44
• Specificity: Recognizes human PDHK1. Species crossreactivity: mouse, rat, monkey.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA,

• Applicable Applications for anti-pdk-1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%; Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:50.
• Immunogen: Synthetic peptide corresponding to the sequence of human PDHK1protein (KLH- coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of extracts from various cell types using MBS606108.)

Related Product Information for anti-pdk-1 antibody

Pyruvate generated from glycolysis is converted to acetyl-CoA by pyruvate dehydrogenase (PDH) under normoxia (1-3). This is a critical link between glycolysis and the TCA cycle (3). PDH activity is regulated by phosphorylation and dephosphorylation (3). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (3). Hypoxia can directly induce pyruvate dehydrogenase kinase 1 (PDHK1) expression, which results in inactivation of PDH and the TCA cycle and subsequent suppression of metabolism (4).

Product Categories/Family for anti-pdk-1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 71992745
• NCBI GeneID: 180475
• NCBI Accession #: NP_001024743.1
• NCBI GenBank Nucleotide #: NM_001029572.2
• UniProt Accession #: Q9Y1J3; Q9UA36
• Molecular Weight: 71,917 Da
• NCBI Official Full Name: Protein PDK-1, isoform b
• NCBI Official Symbol: pdk-1
• NCBI Protein Information: Protein PDK-1
• UniProt Protein Name: 3-phosphoinositide-dependent protein kinase 1
• UniProt Gene Name: pdk-1
• UniProt Entry Name: PDPK1_CAEEL

Uniprot Description

Function: Involved in the daf-2/insulin receptor-like transduction pathway, which controls longevity and prevents developmental arrest at the dauer stage. Phosphorylates and activates sgk-1, akt-1 and akt-2. Ref.1 Ref.3

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Subunit structure: Interacts directly with sgk-1, akt-1 and akt-2. Ref.3

Subcellular location: Cytoplasm

By similarity.

Developmental stage: Expressed in late stage embryos and throughout life. At L1, expressed in neurons, intestinal cells and hypodermal cells. In adults, expressed in the somatic gonad. Ref.1

Sequence similarities: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PDPK1 subfamily.Contains 1 protein kinase domain.