Western Blot (WB)
(Western blot analysis of extracts from mouse and rat brain using MBS605919.)
Rabbit Calmodulin-Dependent Protein Kinase II, pan Monoclonal Antibody | anti-CaMKII antibody
Calmodulin-Dependent Protein Kinase II, pan (CaM Kinase II, CaMKII, CaMK-II, CAMK2, Calcium/calmodulin-dependent Protein Kinase II alpha, Calcium/calmodulin-dependent Protein Kinase Type II alpha, CAMKA, CAMK2A Calcium/calmodulin-dependent Protein Kinase
Gene Names
CaMKII; Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CAMKIId; CDPK1
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Synonyms
Calmodulin-Dependent Protein Kinase II; pan; Monoclonal Antibody; pan (CaM Kinase II; CaMKII; CaMK-II; CAMK2; Calcium/calmodulin-dependent Protein Kinase II alpha; Calcium/calmodulin-dependent Protein Kinase Type II alpha; CAMKA; CAMK2A Calcium/calmodulin-dependent Protein Kinase; Anti -Calmodulin-Dependent Protein Kinase II; anti-CaMKII antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
10E11
Specificity
Recognizes human CaMKII (pan) (D11A10) XPTM. Species crossreactivity: mouse, rat.
Form/Format
Supplied in liquid 10mM HEPES, pH 7.5, 150mM NaCl, 100ug/ml BSA, 50% glycerol, 0.02% sodium azide.
Applicable Applications for anti-CaMKII antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,
0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS,
0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunogen
Synthetic peptide surrounding Val184 of human CaMKII-A (KLH-coupled).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from mouse and rat brain using MBS605919.)
Western Blot (WB)
(Western blot analysis of extracts from mouse and rat brain using MBS605919.)
Related Product Information for anti-CaMKII antibody
CaMKII is an important member of the calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling (1,2). CaMKII has catalytic and regulatory domains. The binding of Ca2+/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase (3). The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active (3). The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1 (4).
Product Categories/Family for anti-CaMKII antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,920 Da
NCBI Official Full Name
Calcium/calmodulin-dependent protein kinase II, isoform J
NCBI Official Synonym Full Names
Calcium/calmodulin-dependent protein kinase II
NCBI Official Symbol
CaMKII
NCBI Official Synonym Symbols
Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CAMKIId; CDPK1
NCBI Protein Information
CG18069 gene product from transcript CG18069-RE; Calcium/calmodulin-dependent protein kinase II, isoform E; CG18069-PE; CaMKII-PE; Ca[2+]/calmodulin-dependent protein kinase II; CaM-kinase-II; calmodulin-dependent kinase; CaM kinase; calmodulin kinase II; calcium/calmodulin-dependent protein kinase II; calcium/ calmodulin-dependent protein kinase II; CaM kinase II; Calcium/calmodulin-dependent protein kinase II, isoform G; CG18069-PG; CaMKII-PG; Calcium/calmodulin-dependent protein kinase II, isoform D; CG18069-PD; CaMKII-PD; Calcium/calmodulin-dependent protein kinase II, isoform I; CG18069-PI; CaMKII-PI; Calcium/calmodulin-dependent protein kinase II, isoform C; CG18069-PC; CaMKII-PC; Calcium/calmodulin-dependent protein kinase II, isoform A; CG18069-PA; CaMKII-PA; Calcium/calmodulin-dependent protein kinase II, isoform B; CG18069-PB; CaMKII-PB; Calcium/calmodulin-dependent protein kinase II, isoform J; CG18069-PJ; CaMKII-PJ; Calcium/calmodulin-dependent protein kinase II, isoform H; CG18069-PH; CaMKII-PH
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type II alpha chain
Protein Family
UniProt Gene Name
CaMKII
UniProt Synonym Gene Names
CaM
UniProt Entry Name
KCC2A_DROME
Uniprot Description
Function: A key regulator of plasticity in synaptic physiology and behavior, alterations in its activity produce pleiotrophic effects that involve synaptic transmission and development as well as various aspects of behavior. Directly modulates eag potassium channels. Ref.6 Ref.7
Catalytic activity: ATP + a protein = ADP + a phosphoprotein.
Enzyme regulation: CASK plays a role in regulation of CaMKII autophosphorylation. When complexed with CASK and in the presence Ca[2+]/CaM, autophosphorylation of Thr-287 causes constitutive activation of the kinase. In the absence of Ca[2+]/CaM, autophosphorylation of Thr-306 causes inactivation of the kinase. Ref.8
Subunit structure: Interacts with CASK. Ref.7
Tissue specificity: Expressed at a high level in the central nervous system during the late embryonic stage. In adults, expression is more abundant in the head than in the body. Ref.1 Ref.2
Post-translational modification: Autophosphorylation at Thr-287 is independent of autophosphorylation at Thr-306 and Thr-307.
Sequence similarities: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.Contains 1 protein kinase domain.
Research Articles on CaMKII
Similar Products
Product Notes
The CaMKII camkii (Catalog #AAA605919) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Calmodulin-Dependent Protein Kinase II, pan (CaM Kinase II, CaMKII, CaMK-II, CAMK2, Calcium/calmodulin-dependent Protein Kinase II alpha, Calcium/calmodulin-dependent Protein Kinase Type II alpha, CAMKA, CAMK2A Calcium/calmodulin-dependent Protein Kinase reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Calmodulin-Dependent Protein Kinase II, pan can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Researchers should empirically determine the suitability of the CaMKII camkii for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calmodulin-Dependent Protein Kinase II, pan, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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