Western Blot (WB)
(Western blot analysis of extracts from various cell lines using MBS621189.)
Rabbit Scribble Polyclonal Antibody | anti-SCRIB antibody
Scribble (Scribbled homolog (Drosophila), CRIB1, hScrib, KIAA0147, LAP4, Protein LAP4, Protein scribble homolog, SCRB1, SCRIB1, Vartul, VARTUL)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1%
Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Western Blot (WB)
(Western blot analysis of extracts from various cell lines using MBS621189.)
Western Blot (WB)
(Western blot analysis of extracts from various cell lines using MBS621189.)
NCBI and Uniprot Product Information
NCBI Description
SCRIB is a cytoplasmic multimodular scaffold protein targeted to epithelial adherens junctions and neuronal presynaptic compartments. SCRIB and its orthologs in vertebrates and invertebrates participate in cell polarization (summary by Nola et al., 2008 [PubMed 18716323]).[supplied by OMIM]
Uniprot Description
Scribble: may be involved in regulating neuroblast cortical polarity, cell size asymmetry and mitotic spindle asymmetry. A neoplastic tumor suppressor genes in Drosophila melanogaster. May play a role in the suppression of mammalian tumorigenesis. Gene ablation in the mouse causes severe neural tube defects. May be implicated in the etiology of human NTDs. Involved in the regulation of synaptic plasticity and synaptic vesicle dynamics. May be required to sustain synaptic vesicle concentrations at their sites of release. Targeted for ubiquitin-mediated degradation by the HPV E6 oncoprotein.
Protein type: Cell adhesion; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: nucleoplasm; presynaptic membrane; postsynaptic membrane; cell-cell adherens junction; lamellipodium; basolateral plasma membrane; leading edge; plasma membrane; intercellular junction
Molecular Function: protein binding
Biological Process: cell migration; viral reproduction; wound healing; positive regulation of apoptosis; establishment of apical/basal cell polarity; cochlear nucleus development; asymmetric protein localization; cell proliferation; cell-cell adhesion; positive chemotaxis; synaptic vesicle endocytosis; neural tube closure; negative regulation of mitotic cell cycle; auditory receptor cell stereocilium organization and biogenesis; synaptic vesicle targeting; positive regulation of receptor recycling; astrocyte cell migration
Research Articles on SCRIB
2. The upregulation of vimentin expression during epithelial to mesenchymal transitions may stabilize Scrib to promote directed cell migration.
3. Results identify MCC as a potential scaffold protein regulating cell movement and able to bind Scrib, beta-catenin and NHERF1/2.
4. Study demonstrates that scribble inhibits breast cancer formation and that deregulation of polarity pathways promotes dysplastic and neoplastic growth in mammals by disrupting morphogenesis and inhibiting cell death.
5. data underscore the role of Scrib in cell migration and show the strong impact of Scrib in the function of PAK and Rac, two key molecules implicated in this process
6. Tax1 induces aberrant clustering of Scribble, thereby altering the functions in HTLV-1-infected cells.
7. Scribble is an important mediator of MAPK signalling and cooperates with H-ras to promote cell invasion.
8. The association of hScrib with APC would be important for mediating the cell cycle regulations.
9. Cleavage of Scrib by executioner caspases is a critical step for detachment of cell contact during the process of apoptosis.
10. this study demonstrates a novel interaction between TBEV NS5 and the PDZ protein scribble (hScrib) affecting interferon (IFN) type I and II mediated JAK-STAT signalling.
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Product Notes
The SCRIB scrib (Catalog #AAA621189) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Scribble (Scribbled homolog (Drosophila), CRIB1, hScrib, KIAA0147, LAP4, Protein LAP4, Protein scribble homolog, SCRB1, SCRIB1, Vartul, VARTUL) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's Scribble can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% w/v BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the SCRIB scrib for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Scribble, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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