Mouse Arrestin 1, beta Monoclonal Antibody | anti-ARRB1 antibody

Arrestin 1, beta, phosphorylated (Ser412) (ARRB1, ARB1, ARR1)

Cat. Num. AAA601033

• Gene Names: ARRB1; ARB1; ARR1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Purified; Purified.
• Synonyms: Arrestin 1; beta; Monoclonal Antibody; phosphorylated (Ser412) (ARRB1; ARB1; ARR1); Anti -Arrestin 1; anti-ARRB1 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A14
• Specificity: Detects endogenous beta-arrestin 1 only when phosphorylated at Ser412. Does not crossreact with beta-arrestin 2. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Purified; Purified.
• Form/Format: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.

• Applicable Applications for anti-ARRB1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blotting and Immunoprecipitation.; Dilution: Western Blotting: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide (KLH coupled) corresponding to residues surrounding Ser412 of human beta-arrestin 1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of HEK293 cells alone (-), expressing beta-arrestin 1 (wt) or beta-arrestin (Ser412Ala) mutant (Ala412), using MBS601033. Lanes 1 and 3 show endogenous levels of phosphorylated beta-arrestin 1.)

Immunoprecipitation (IP)

(Immunoprecipitation of beta-arrestin 1 from HEK293 cells using a polyclonal antibody to MBS601033, followed by alkaline phosphaphatase (CIP) treatment. Beta-arrestin 1 was detected by MBS601033. CIP treatment abolished the beta-arrestin 1 signal, indicating that the mono clonal antibody is phospho-specific.)

Related Product Information for anti-ARRB1 antibody

Beta-arrestin 1 is a member of the arrestin family, which has been implicated in mediating the desensitization and internalization of G protein-coupled receptors (GPCR) (1). Beta-arrestin 1 is constitutively phosphorylated by Erk1/2 at serine 412 of the C-terminus and localized in the cytosol (2). When recruited to the plasma membrane after agonist stimulation of the beta2-adrenergic receptors, beta-arrestin 1 is rapidly dephosphorylated. This dephosphorylation is an essential step of beta-arrestin 1-mediated receptor endocytosis, but it is not required for receptor desensitization (3).

Product Categories/Family for anti-ARRB1 antibody

Antibodies; Abs to Arrestin

NCBI and Uniprot Product Information

• NCBI GI #: 209944101
• NCBI GeneID: 408
• UniProt Accession #: P49407; O75625; O75630; Q2PP20; Q9BTK8; B6V9G8
• Molecular Weight: 47,066 Da
• NCBI Official Full Name: arrestin beta 1 transcript variant 2
• NCBI Official Synonym Full Names: arrestin, beta 1
• NCBI Official Symbol: ARRB1
• NCBI Official Synonym Symbols: ARB1; ARR1
• NCBI Protein Information: beta-arrestin-1; arrestin 2
• UniProt Protein Name: Beta-arrestin-1
• Protein Family: Beta-arrestin
• UniProt Gene Name: ARRB1
• UniProt Synonym Gene Names: ARR1
• UniProt Entry Name: ARRB1_HUMAN

NCBI Description

Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described. [provided by RefSeq, Jan 2011]

Uniprot Description

ARRB1: regulates G-protein coupled receptors (GPCR) signaling by mediating both receptor desensitization and resensitization processes. Binds to GRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; the binding appears to require receptor determinants exposed only in the active receptor conformation. Targets many receptors for internalization by acting as endocytic adapters (CLASPs, clathrin-associated sorting proteins). Internalized arrestin-receptor complexes traffic to intracellular endosomes, where they remain uncoupled from G-proteins. Two different modes of arrestin-mediated internalization occur. Beta-arrestins function as multivalent adapter proteins that can switch the GPCR from a G-protein signaling mode that transmits short-lived signals from the plasma membrane via small molecule second messengers and ion channels to a beta-arrestin signaling mode that transmits a distinct set of signals that are initiated as the receptor internalizes and transits the intracellular compartment. Also involved in regulation of receptors other than GPCRs. Involved in Toll-like receptor and IL-1 receptor signaling through the interaction with TRAF6 which prevents TRAF6 autoubiquitination and oligomerization required for activation of NF-kappa-B and JUN. Binds phosphoinositides. Binds inositolhexakisphosphate (InsP6). Involved in IL8-mediated granule release in neutrophils. Interacts with phosphorylated ADRB2 and CHRM2. Interacts with SRC (via the SH3 domain and the protein kinase domain); the interaction is independent of the phosphorylation state of SRC C-terminus. Interacts with RAF1, CHUK, IKBKB and Nik. Interacts with DVL1 and DVL2; the interaction is enhanced by DVL phosphorylation. Interacts with IGF1R. Belongs to the arrestin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; Golgi membrane; cytoplasmic vesicle membrane; lysosomal membrane; cytoplasm; plasma membrane; heterotrimeric G-protein complex; coated pit; pseudopodium; cytoplasmic vesicle; nucleus; chromatin; cytosol

Molecular Function: angiotensin receptor binding; enzyme inhibitor activity; insulin-like growth factor receptor binding; protein binding; histone acetyltransferase activity; mitogen-activated protein kinase kinase binding; ubiquitin protein ligase binding; caspase inhibitor activity; transcription factor binding; GTPase activator activity

Biological Process: transcription from RNA polymerase II promoter; platelet activation; proteasomal ubiquitin-dependent protein catabolic process; Notch signaling pathway; positive regulation of protein binding; positive regulation of receptor internalization; positive regulation of Rho protein signal transduction; protein ubiquitination; negative regulation of caspase activity; positive regulation of peptidyl-serine phosphorylation; protein transport; G-protein coupled receptor internalization; inhibition of NF-kappaB transcription factor; phototransduction; negative regulation of interleukin-6 production; stress fiber formation; negative regulation of interleukin-8 production; positive regulation of histone acetylation; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation; post-Golgi vesicle-mediated transport; blood coagulation; positive regulation of GTPase activity