Rabbit anti-Human CD98 Polyclonal Antibody | anti-SLC3A2 antibody

Anti-CD98 Antibody, Rabbit Polyclonal

Cat. Num. AAA8106279

• Gene Names: SLC3A2; 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CD98; Polyclonal Antibody; Anti-CD98 Antibody; Rabbit Polyclonal; CD98/SLC3A2 Antibody; Rabbit PAb; Antigen Affinity Purified; solute carrier family 3 (amino acid transporter heavy chain); member 2; Anti-4F2 Antibody; Anti-4F2HC Antibody; Anti-4T2HC Antibody; Anti-CD98HC Antibody; Anti-MDU1 Antibody; Anti-NACAE Antibody; anti-SLC3A2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CD98/SLC3A2
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SLC3A2 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:2000; ICC/IF: 1:100-1:500; IP: 5-10uL/mg of lysate
• Immunogen: Recombinant Human CD98/SLC3A2 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD98/SLC3A2 (rh CD98/SLC3A2). CD98/SLC3A2 specific IgG was purified by Human CD98/SLC3A2 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CD98 in human kidney with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human CD98 in human placenta with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

Immunofluorescence (IF)

(Immunofluorescence staining of CD98 in MCF7 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-Human CD98 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm and cell membrane.)

Immunoprecipitation (IP)

(SLC3A2 was immunoprecipitated using: Lane A:0.5 mg HEPG2 Whole Cell Lysate 4 uL anti-SLC3A2 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-SLC3A2 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 72 kDa Observed band size :72 kDa)

Western Blot (WB)

(Anti-CD98 rabbit polyclonal antibody at 1:500 dilution Lane A: HeLa Whole Cell Lysate Lane B: HepG2 Whole Cell Lysate Lane C: Raji Whole Cell Lysate Lane D: Molt-4 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the technique. Performed under reducing conditions. Predicted band size:68 kDa Observed band size:75 kDa (We are unsure as to the identity of these extra bands.))

Related Product Information for anti-SLC3A2 antibody

4F2 cell-surface antigen heavy chain, also known as 4F2 heavy chain antigen, Lymphocyte activation antigen 4F2 large subunit, CD98, SLC3A2 and MDU1, is a single-pass type I I membrane protein that belongs to the SLC3A transporter family. SLC3A2/MDU1 is expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta is significantly stronger at full-term than at the mid-trimester stage. SLC3A2/MDU1 is expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. It is expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. SLC3A2/MDU1 is also expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco2-BBE. SLC3A2/MDU1 is required for the function of light chain amino-acid transporters. It is involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. SLC3A2/MDU1 is involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7, SLC3A2/MDU1 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. SLC3A2/MDU1 plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. It is required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, SLC3A2/MDU1 is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta.

References

Torrents D. et al., 1998, J Biol Chem. 273: 32437-45. Pfeiffer R. et al., 1999, EMBO J. 18: 49-57. Broeer A. et al., 2000, Biochem J. 349: 787-95. Yanagida O. et al., 2001, Biochim Biophys Acta. 1514: 291-302. Broeer A. et al., 2001, Biochem J. 355: 725-31. Fort J. et al., 2007, J Biol Chem. 282: 31444-52. Mayya V. et al., 2009, Sci Signal. 2: RA46-RA46.

NCBI and Uniprot Product Information

• NCBI GI #: 257051063
• NCBI GeneID: 6520
• UniProt Accession #: P08195; Q13543
• Molecular Weight: 71,123 Da
• NCBI Official Full Name: 4F2 cell-surface antigen heavy chain
• NCBI Official Synonym Full Names: solute carrier family 3 member 2
• NCBI Official Symbol: SLC3A2
• NCBI Official Synonym Symbols: 4F2; CD98; MDU1; 4F2HC; 4T2HC; NACAE; CD98HC
• NCBI Protein Information: 4F2 cell-surface antigen heavy chain
• UniProt Protein Name: 4F2 cell-surface antigen heavy chain
• Protein Family: 4F2 cell-surface antigen
• UniProt Gene Name: SLC3A2
• UniProt Synonym Gene Names: MDU1; 4F2hc

NCBI Description

This gene is a member of the solute carrier family and encodes a cell surface, transmembrane protein. The protein exists as the heavy chain of a heterodimer, covalently bound through di-sulfide bonds to one of several possible light chains. The encoded transporter plays a role in regulation of intracellular calcium levels and transports L-type amino acids. Alternatively spliced transcript variants, encoding different isoforms, have been characterized. [provided by RefSeq, Nov 2010]

Uniprot Description

Required for the function of light chain amino-acid transporters. Involved in sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan. Involved in guiding and targeting of LAT1 and LAT2 to the plasma membrane. When associated with SLC7A6 or SLC7A7 acts as an arginine/glutamine exchanger, following an antiport mechanism for amino acid transport, influencing arginine release in exchange for extracellular amino acids. Plays a role in nitric oxide synthesis in human umbilical vein endothelial cells (HUVECs) via transport of L-arginine. Required for normal and neoplastic cell growth. When associated with SLC7A5/LAT1, is also involved in the transport of L-DOPA across the blood-brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. When associated with SLC7A5 or SLC7A8, involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the transmembrane. Together with ICAM1, regulates the transport activity LAT2 in polarized intestinal cells, by generating and delivering intracellular signals. When associated with SLC7A5, plays an important role in transporting L-leucine from the circulating blood to the retina across the inner blood-retinal barrier. When associated with LAPTM4B, recruits SLC3A2 and SLC7A5 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (PubMed:25998567).