Mouse Nitrotyrosine Monoclonal Antibody | anti-NOS2 antibody

Nitrotyrosine (nTyr)

Cat. Num. AAA603220

• Gene Names: NOS2; iNOS; NOS2A
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by thiophilic adsorption and size exclusion chromatography.
• Synonyms: Nitrotyrosine; Monoclonal Antibody; Nitrotyrosine (nTyr); Anti -Nitrotyrosine (nTyr); anti-NOS2 antibody

• Host: Mouse
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 6D609
• Specificity: Recognizes Nitrotyrosine (nTyr). Species Crossreactivity: human, mouse, rat and canine
• Purity/Purification: Purified; Purified by thiophilic adsorption and size exclusion chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, PEG, sucrose, 0.1% sodium azide. Reconstitute in 1ml sterile ddH2O.

• Applicable Applications for anti-NOS2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.05ug/ml; Western Blot: 1ug/ml for AP/BCIP/NBT (MTT detection) or 0.1ug/ml for HRPO/ECL detection.
• Immunogen: KLH modified with nitrotyrosine
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NOS2 antibody

The enzymatic conversion of arginine to citrulline by nitric oxide synthase (NO Synthase) leads to the formation of nitric oxide (NO). NO forms peroxynitrite following the reaction with superoxide. Peroxynitrite is a potent oxidant that reacts with a variety of biological molecules, including tyrosine residues in proteins. Tyrosine nitration can inactivate enzymes and receptors that depend on tyrosine residues for their activity. Nitration prevents phosphorylation of tyrosnine residues important for signal transduction.

Product Categories/Family for anti-NOS2 antibody

Antibodies; Abs to Nitrotyrosine

NCBI and Uniprot Product Information

• NCBI GI #: 116003827
• NCBI GeneID: 282876
• NCBI Accession #: NP_001070267.1
• NCBI GenBank Nucleotide #: NM_001076799.1
• UniProt Accession #: Q27995; Q06Q84; Q27985
• Molecular Weight: 131,208 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: iNOS; NOS2A
• NCBI Protein Information: nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: Inducible NOS; iNOS; NOSII
• UniProt Entry Name: NOS2_BOVIN

Uniprot Description

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2

By similarity.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Regulated by calcium/calmodulin

By similarity.

Subunit structure: Homodimer. Binds SLC9A3R1

By similarity.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.