Rabbit anti-Rat iNOS Polyclonal Antibody | anti-iNOS antibody

iNOS polyclonal antibody

Cat. Num. AAA3013813

• Gene Names: Nos2; iNOS; Nos-2; Nos2a; i-NOS; NOS-II
• Reactivity: Rat
• Applications: Immunohistochemistry
• Purity: affinity purified by Protein A
• Synonyms: iNOS; Polyclonal Antibody; iNOS polyclonal antibody; i NOS; Nitric Oxide Synthase; Inducible; HEP NOS; Hepatocyte NOS; HEPNOS; Inducible nitric oxide synthase; Inducible NO synthase; Inducible NOS; INOS; Inosl; MAC NOS; Macrophage NOS; Nitric oxide synthase 2 inducible macrophage; Nitric oxide synthase 2A (inducible hepatocytes); Nitric oxide synthase inducible; NOS 2; NOS 2A; NOS; Nos II; NOS type II; Nos2; NOS2A; NOS2_HUMAN; anti-iNOS antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: iNOS polyclonal Antibody detects endogenous levels of iNOS protein.
• Purity/Purification: affinity purified by Protein A
• Form/Format: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• Applicable Applications for anti-iNOS antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:400-800
• Immunogen: KLH conjugated synthetic peptide derived from mouse iNOS:811-910/1153
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (iNOS) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C.)

Related Product Information for anti-iNOS antibody

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. All NOS isoforms contain calmodulin, nicotinamide adenine dinucleotide phosphate (NADPH), flavin adenine dinucleotide (FAD), and flavin mononucleotide (FMN) binding domains. Nitric oxide synthase is expressed in liver, macrophages, hepatocytes, synoviocytes, stimulated glial cells and smooth muscle cells. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. After cytokine induction, iNOS exhibits a delayed activity response which is then followed by a significant increase in NO production over a long period of time. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

NCBI and Uniprot Product Information

• NCBI GI #: 6754872
• NCBI GeneID: 18126
• NCBI Accession #: NP_035057.1
• NCBI GenBank Nucleotide #: NM_010927.3
• UniProt Accession #: P29477; O70515; O70516; Q5SXT3; Q6P6A0; Q8R410
• Molecular Weight: 130,575 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: Nos2
• NCBI Official Synonym Symbols: iNOS; Nos-2; Nos2a; i-NOS; NOS-II
• NCBI Protein Information: nitric oxide synthase, inducible; MAC-NOS; NOS type II; inducible NO synthase; inducible NOS; inducible nitric oxide synthase; macrophage NOS; nitric oxide synthase 2, inducible, macrophage; peptidyl-cysteine S-nitrosylase NOS2
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Inositol 3-kinase
• UniProt Gene Name: Nos2
• UniProt Synonym Gene Names: Inosl; Inducible NOS; iNOS; MAC-NOS
• UniProt Entry Name: NOS2_MOUSE

NCBI Description

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Uniprot Description

iNOS: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2. Homodimer. Binds SLC9A3R1. By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta. Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets. Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and effects may be exerted at the level of translational/post-translational modification and directly on the catalytic activity. Belongs to the NOS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.14.13.39; Amino Acid Metabolism - arginine and proline; Oxidoreductase

Cellular Component: cortical cytoskeleton; extracellular space; perinuclear region of cytoplasm; cytoplasm; peroxisome; intracellular; vesicle membrane; cytosol; nucleus

Molecular Function: protein homodimerization activity; FAD binding; nitric-oxide synthase activity; metal ion binding; beta-catenin binding; cAMP-dependent protein kinase regulator activity; oxidoreductase activity; Hsp90 protein binding; actin binding; protein kinase binding; calmodulin binding; protein binding; NADPH-hemoprotein reductase activity; FMN binding; cadherin binding; iron ion binding; heme binding; nitric-oxide synthase binding; NADP binding; receptor binding

Biological Process: superoxide metabolic process; response to lipopolysaccharide; positive regulation of guanylate cyclase activity; positive regulation of killing of cells of another organism; positive regulation of vasodilation; ovulation from ovarian follicle; regulation of cell proliferation; defense response to bacterium; response to hypoxia; peptidyl-cysteine S-nitrosylation; arginine catabolic process; negative regulation of blood pressure; negative regulation of protein catabolic process; nitric oxide biosynthetic process; inflammatory response; regulation of protein kinase activity; regulation of insulin secretion; nitric oxide mediated signal transduction