Rabbit Nitrotyrosine Polyclonal Antibody | anti-NOS2 antibody

Nitrotyrosine (nTyr)

Cat. Num. AAA612648

• Gene Names: NOS2; iNOS; NOS2A
• Applications: Western Blot
• Synonyms: Nitrotyrosine; Polyclonal Antibody; Nitrotyrosine (nTyr); Anti -Nitrotyrosine (nTyr); anti-NOS2 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Specificity: Recognizes nitrotyrosine; less than 10% reactivity with chlorotyrosine. Does not cross-react with synthetic peptides containing unmodified tyrosine.
• Form/Format: Supplied as a liquid in TBS, pH 7.4, containing 0.5mg/ml BSA, 50% glycerol and 0.02% sodium azide.

• Applicable Applications for anti-NOS2 antibody: Western Blot (WB)
• Application Notes: Western Blot: 2ug/ml.
• Immunogen: Nitrotyrosine-containing synthetic peptide.
• Preparation and Storage: -20 degree C

Western Blot (WB)

(Western Blot of MBS612648)

Product Categories/Family for anti-NOS2 antibody

Antibodies; Abs to Nitrotyrosine

NCBI and Uniprot Product Information

• NCBI GI #: 116003827
• NCBI GeneID: 282876
• NCBI Accession #: NP_001070267.1
• NCBI GenBank Nucleotide #: NM_001076799.1
• UniProt Accession #: Q27995; Q06Q84; Q27985
• Molecular Weight: 131,208 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible<
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: iNOS; NOS2A
• NCBI Protein Information: nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: Inducible NOS; iNOS; NOSII
• UniProt Entry Name: NOS2_BOVIN

Uniprot Description

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2

By similarity.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Regulated by calcium/calmodulin

By similarity.

Subunit structure: Homodimer. Binds SLC9A3R1

By similarity.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.