Goat Nitrotyrosine Polyclonal Antibody | anti-NOS2 antibody

Nitrotyrosine (nTyr)

Cat. Num. AAA622995

• Gene Names: NOS2; iNOS; NOS2A
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purififed by Ig salt fraction.
• Synonyms: Nitrotyrosine; Polyclonal Antibody; Nitrotyrosine (nTyr); Anti -Nitrotyrosine (nTyr); anti-NOS2 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes free Nitrotyrosine and nitrotyrosine-containing proteins.
• Purity/Purification: Purififed by Ig salt fraction.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.
• Concentration: 40 mg/ml (Total protein). (varies by lot)

• Applicable Applications for anti-NOS2 antibody: Suitable for use in ELISA (EIA), Western Blot (WB) and Immunohistochemistry (IHC). Other applications not tested.
• Application Notes: Western Blot 1:1000; Immunohistochemistry: 1:100-1:10,000; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Nitrated KLH
• Positive Control: MBS657779: Nitrotyrosine, Immunoblot Positive Control (nTyr).
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

ELISA (EIA)

(Nitrotyrosine antibody specificity. Competition for MBS622995 binding to a NT containing 13mer peptide coated on a plate. ELISA competitors are free nitrotyrosine (diamond), NT containing 33mer peptide (circle), chlorotyrosine (square), and tyrosine (triangle). Competition for Ab binding was most effective with free NT followed closely by the NT containing 33mer peptide. This shows that MBS622995 will recognize free NT as well as NT containing proteins in biological fluids. Significant competition by tyrosine and modified tyrosine occurred at greater than two orders of magnitude higher concentrations of each, indicating very good NT Ab specificity.)

Western Blot (WB)

(Western Blot using nitrotyrosine immunoblot positive control on nitrocellulose with MBS622995 at 1:1000 dilution. Lane 1, 2.44ug; Lane 2, 9.75ng positive control (nitrated carbonic anhydrase).)

Related Product Information for anti-NOS2 antibody

The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates. Nitrotyrosine can be detected and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Product Categories/Family for anti-NOS2 antibody

Antibodies; Abs to Nitrotyrosine

NCBI and Uniprot Product Information

• NCBI GI #: 116003827
• NCBI GeneID: 282876
• NCBI Accession #: NP_001070267.1
• NCBI GenBank Nucleotide #: NM_001076799.1
• UniProt Accession #: Q27995; Q06Q84; Q27985
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible<
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: iNOS; NOS2A
• NCBI Protein Information: nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: Inducible NOS; iNOS; NOSII
• UniProt Entry Name: NOS2_BOVIN

Uniprot Description

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2

By similarity.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Regulated by calcium/calmodulin

By similarity.

Subunit structure: Homodimer. Binds SLC9A3R1

By similarity.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.