Rabbit anti-Human, Mouse Nitrotyrosine Polyclonal Antibody | anti-NOS2 antibody

Nitrotyrosine (nTyr)

Cat. Num. AAA612511

• Gene Names: NOS2; iNOS; NOS2A
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified by Protein A affinity chromatography.
• Synonyms: Nitrotyrosine; Polyclonal Antibody; Nitrotyrosine (nTyr); Anti -Nitrotyrosine (nTyr); anti-NOS2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes nitrated tyrosine residues
• Purity/Purification: Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in buffer, 0.1% sodium azide.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-NOS2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Recommended Dilutions:; Western Blot: 1:100-1:1000 when tested against nitrated BSA and nitrated ovalbumin.; Immunohistochemistry (paraffin): 1:500 mouse brain (animal treated with Zymosan) with pretreatment with citrate buffer, pH 6.0.
• Immunogen: Nitrated KLH
• Grade: Affinity Purified
• Positive Control: Tyrosine residues can be nitrated directly in situ on tissue sections using peroxynitrite to create a positive control. After the tissue section is deparaffinized, the slide is covered with a drop of PBS, pH 7.4 into which 10ul of peroxynitrite solution are mixed. Care should be taken that the solution maintains a pH of 7.4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Nytrotyrosine staining of Mouse Brain (Zymosan), tissue pretreated with citrate buffer, pH 6.0. A 1:500 dilution of MBS612511 was used followed by HRP/DAB staining. Immunoreactivity is seen in the hippocampus.)

Immunohistochemistry (IHC)

(Negative control staining of normal cerebellum, tissue pretreated with citrate buffer, pH 6.0, Negative Control Reagent, IHC Select® detection with MBS612511. No signal detected.)

Immunohistochemistry (IHC)

(Nitrotyrosine staining of polyclonal normal cerebellum, tissue pretreated with citrate buffer, pH 6.0, pAb at 1:500 overnight incubation, IHC Select® detection with MBS612511. Immunoreactivity is seen in the neurons)

Immunohistochemistry (IHC)

(Negative control staining of normal cerebellum, tissue pretreated with citrate buffer, pH 6.0, Negative Control Reagent, IHC Select® detection with MBS612511 No signal detected.)

Related Product Information for anti-NOS2 antibody

The cellular production of highly reactive nitrogen species derived from nitric oxide, such as peroxynitrite, nitrogen dioxide and nitryl chloride, leads to the nitration of tyrosine resides in tissue proteins. The extent of protein nitrotyrosine formation provides an index of the production of reactive nitrogen species and potential cell damage over a period of time. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected, estimated semi-quantitatively and located in cells and tissues by immunocytochemical techniques using antibodies directed against the nitrotyrosine hapten.

Product Categories/Family for anti-NOS2 antibody

Antibodies; Abs to Nitrotyrosine

NCBI and Uniprot Product Information

• NCBI GI #: 116003827
• NCBI GeneID: 282876
• NCBI Accession #: NP_001070267.1
• NCBI GenBank Nucleotide #: NM_001076799.1
• UniProt Accession #: Q27995; Q06Q84; Q27985
• Molecular Weight: 131,208 Da
• NCBI Official Full Name: nitric oxide synthase, inducible
• NCBI Official Synonym Full Names: nitric oxide synthase 2, inducible
• NCBI Official Symbol: NOS2
• NCBI Official Synonym Symbols: iNOS; NOS2A
• NCBI Protein Information: nitric oxide synthase, inducible; NOSII; NOS type II; inducible NOS; inducible NO synthase; inducible nitric oxide synthase; peptidyl-cysteine S-nitrosylase NOS2; nitric oxide synthase 2A (inducible, hepatocytes)
• UniProt Protein Name: Nitric oxide synthase, inducible
• Protein Family: Nitric oxide synthase
• UniProt Gene Name: NOS2
• UniProt Synonym Gene Names: Inducible NOS; iNOS; NOSII
• UniProt Entry Name: NOS2_BOVIN

Uniprot Description

Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2

By similarity.

Catalytic activity: 2 L-arginine + 3 NADPH + 4 O2 = 2 L-citrulline + 2 nitric oxide + 3 NADP+ + 4 H2O.

Cofactor: Heme group

By similarity.Binds 1 FAD

By similarity.Binds 1 FMN

By similarity.Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme

By similarity.

Enzyme regulation: Regulated by calcium/calmodulin

By similarity.

Subunit structure: Homodimer. Binds SLC9A3R1

By similarity.

Sequence similarities: Belongs to the NOS family.Contains 1 FAD-binding FR-type domain.Contains 1 flavodoxin-like domain.