Rabbit ECE2 Polyclonal Antibody | anti-ECE2 antibody

Anti-ECE2 Antibody

Cat. Num. AAA416221

• Reactivity: Human, Rat. Predicted: Mouse.
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: ECE2; Polyclonal Antibody; Anti-ECE2 Antibody; anti-ECE2 antibody

• Host: Rabbit
• Reactivity: Human, Rat. Predicted: Mouse.
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 765

• Applicable Applications for anti-ECE2 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:500-1:1,000; Immunohistochemistry: 1:100-1:500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal ECE2 (1) antibody was raised against a recombinate human ECE2 protein 23-252 aa (BC005835).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

Related Product Information for anti-ECE2 antibody

This gene encodes a member of the M13 family, which includes type 2 integral membrane metallopeptidases. The encoded enzyme is a membrane-bound zinc-dependent metalloprotease. The enzyme catalyzes the cleavage of big endothelin to produce the vasoconstrictor endothelin-1, and plays a role in the processing of several neuroendocrine peptides. It may also have methyltransferase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

References

(1) Elks,C.E., Perry,J.R., Sulem,P. et al. Thirty new loci for age at menarche identified by a meta-analysis of genome-wide association studies. Nat. Genet. 42 (12), 1077-1085 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 153945836
• NCBI GeneID: 9718
• NCBI Accession #: NP_001093591.1
• NCBI GenBank Nucleotide #: NM_001100121.1
• Molecular Weight: 91,211 Da
• NCBI Official Full Name: endothelin-converting enzyme 2 isoform E
• NCBI Official Synonym Full Names: endothelin converting enzyme 2
• NCBI Official Symbol: ECE2
• NCBI Protein Information: endothelin-converting enzyme 2
• UniProt Protein Name: Endothelin-converting enzyme 2
• Protein Family: Endothelin-converting enzyme
• UniProt Gene Name: ECE2
• UniProt Entry Name: ECE2_HUMAN

NCBI Description

This gene encodes a member of the M13 family, which includes type 2 integral membrane metallopeptidases. The encoded enzyme is a membrane-bound zinc-dependent metalloprotease. The enzyme catalyzes the cleavage of big endothelin to produce the vasoconstrictor endothelin-1, and plays a role in the processing of several neuroendocrine peptides. It may also have methyltransferase activity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]

Uniprot Description

ECE2: Converts big endothelin-1 to endothelin-1. Also involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. May limit beta- amyloid peptide accumulation in brain. May also have methyltransferase activity. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.24.71; Protease; Membrane protein, integral; Vesicle; Methyltransferase; Methyltransferase, protein arginine, predicted

Chromosomal Location of Human Ortholog: 3q27.1

Cellular Component: Golgi membrane; cytoplasmic vesicle membrane; integral to membrane

Molecular Function: methyltransferase activity; metal ion binding; metalloendopeptidase activity

Biological Process: methylation; cardioblast differentiation; cell-cell signaling; heart development; peptide hormone processing; brain development; proteolysis