Western Blot (WB)
(NEFL rabbit polyclonal antibody. Western Blot analysis of NEFL expression in Hela NE.)
Rabbit anti-Human Neurofilament L, 68kD Polyclonal Antibody
Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, CMT1F, CMT2E, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein)
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
SerumSerum
Synonyms
Neurofilament L; 68kD; Polyclonal Antibody; 68kD (NF-L; NFL; 68kD Neurofilament Protein; CMT1F; CMT2E; NEFL; Neurofilament Light Polypeptide 68kD; NF68; Neurofilament Triplet L Protein); Anti -Neurofilament L; anti-Neurofilament L; 68kD antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human NEFL.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid.
Sequence
MSSFSYEPYYSTSYKRRYVETPRVHISSVRSGYSTARSAYSSYSAPVSSSLSVRRSYSSSSGSLMPSLENLDLSQVAAISNDLKSIRTQEKAQLQDLNDRFASFIERVHELEQQNKVLEAELLVLRQKHSEPSRFRALYEQEIRDLRLAAEDATNEKQALQGEREGLEETLRNLQARYEEEVLSREDAEGRLMEARKGADEAALARAELEKRIDSLMDEISFLKKVHEEEIAELQAQIQYAQISVEMDVTKPDLSAALKDIRAQYEKLAAKNMQNAEEWFKSRFTVLTESAAKNTDAVRAAKDEVSESRRLLKAKTLEIEACRGMNEALEKQLQELEDKQNADISAMQDTINKLENELRTTKSEMARYLKEYQDLLNVKMALDIEIAAYRKLLEGEETRLSFTSVGSITSGYSQSSQVFGRSAYGGLQTSSYLMSTRSFPSYYTSHVQEEQIEVEETIEAAKAEEAKDEPPSEGEAEEEEKDKEEAEEEEAAEEEEAAKEESEEAKEEEEGGEGEEGEETKEAEEEEKKVEGAGEEQAAKKKD
Applicable Applications for anti-Neurofilament L, 68kD antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Immunogen
Full length human NEFL, aa1-543 (NP_006149.2).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(NEFL rabbit polyclonal antibody. Western Blot analysis of NEFL expression in Hela NE.)
Western Blot (WB)
(NEFL rabbit polyclonal antibody. Western Blot analysis of NEFL expression in Hela NE.)
Western Blot (WB)
(Western Blot analysis of NEFL expression in transfected 293T cell line by NEFL polyclonal antibody. Lane 1: NEFL transfected lysate (61.5kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of NEFL expression in transfected 293T cell line by NEFL polyclonal antibody. Lane 1: NEFL transfected lysate (61.5kD). Lane 2: Non-transfected lysate.)
Immunoprecipitation (IP)
(Immunoprecipitation of NEFL transfected lysate using NEFL rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEFL purified mouse polyclonal antibody)
Immunoprecipitation (IP)
(Immunoprecipitation of NEFL transfected lysate using NEFL rabbit polyclonal antibody and Protein A Magnetic Bead, and immunoblotted with NEFL purified mouse polyclonal antibody)
Related Product Information for anti-Neurofilament L, 68kD antibody
Neurofilaments are the intermediate filaments found in neuronal cells. They are composed of a combination of subunit proteins which typically includes the neurofilment triplet proteins, NF-L (68kD), NF-M (160kD), and NF-H (200kD). Neurofilaments may also include peripherin, alpha-internexin (NF66), nestin, and vimentin. Antibodies to the various neurofilament subunits are useful biomarkers; the filaments are abundant and biochemically stable. The expression of neurofilament proteins varies during neuronal development and differentiation and between between neuronal cell subtypes, and altered in pathological states. Accumulation of neurofilaments is observed the progression of numerous neurological diseases.
Product Categories/Family for anti-Neurofilament L, 68kD antibody
Similar Products
Product Notes
The Neurofilament L, 68kD (Catalog #AAA6000777) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Neurofilament L, 68kD (NF-L, NFL, 68kD Neurofilament Protein, CMT1F, CMT2E, NEFL, Neurofilament Light Polypeptide 68kD, NF68, Neurofilament Triplet L Protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Neurofilament L, 68kD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Researchers should empirically determine the suitability of the Neurofilament L, 68kD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MSSFSYEPYY STSYKRRYVE TPRVHISSVR SGYSTARSAY SSYSAPVSSS LSVRRSYSSS SGSLMPSLEN LDLSQVAAIS NDLKSIRTQE KAQLQDLNDR FASFIERVHE LEQQNKVLEA ELLVLRQKHS EPSRFRALYE QEIRDLRLAA EDATNEKQAL QGEREGLEET LRNLQARYEE EVLSREDAEG RLMEARKGAD EAALARAELE KRIDSLMDEI SFLKKVHEEE IAELQAQIQY AQISVEMDVT KPDLSAALKD IRAQYEKLAA KNMQNAEEWF KSRFTVLTES AAKNTDAVRA AKDEVSESRR LLKAKTLEIE ACRGMNEALE KQLQELEDKQ NADISAMQDT INKLENELRT TKSEMARYLK EYQDLLNVKM ALDIEIAAYR KLLEGEETRL SFTSVGSITS GYSQSSQVFG RSAYGGLQTS SYLMSTRSFP SYYTSHVQEE QIEVEETIEA AKAEEAKDEP PSEGEAEEEE KDKEEAEEEE AAEEEEAAKE ESEEAKEEEE GGEGEEGEET KEAEEEEKKV EGAGEEQAAK KKD. It is sometimes possible for the material contained within the vial of "Neurofilament L, 68kD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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