Immunohistochemistry (IHC)
(Nestin staining of mouse pancreas using MBS600695. Tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25ug/ml, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.)
Mouse Nestin Monoclonal Antibody | anti-NES antibody
Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes)
Purified
Purified
Dilution: Immunohistochemistry (Frozen or paraffin): 1:20-1:200. Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM.
Western Blot: 200-220kD in reducing gels of newborn rat or mouse cell extracts. Prepare samples by boiling in 4 volumes of 125mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol, 4.6% SDS. 5% PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -70 degree C will show breakdown of nestin sometimes into multiple fragments.
Immunocytochemistry: 1:20-1:200. Fix cells in 4% paraformaldehyde buffered with 50mM sodium borate, pH 9.5.
Immunohistochemistry (IHC)
(Nestin staining of mouse pancreas using MBS600695. Tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25ug/ml, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.)
Immunohistochemistry (IHC)
(Nestin staining of mouse pancreas using MBS600695. Tissue was pretreated with citrate buffer, pH 6.0. A previous lot of this antibody was diluted to 1.25ug/ml, using IHC-Select detection with HRP-DAB. Immunoreactivity is detected in the islets of Langerhans.)
Immunohistochemistry (IHC)
(Negative control staining of mouse pancreas using MBS600695. Tissue pretreated with citrate buffer, pH 6.0. Negative Control Reagent, using IHC-Select Detection with HRP-DAB. No signal detected.)
Immunohistochemistry (IHC)
(Negative control staining of mouse pancreas using MBS600695. Tissue pretreated with citrate buffer, pH 6.0. Negative Control Reagent, using IHC-Select Detection with HRP-DAB. No signal detected.)
Testing Data
(Immunolocalization of Nestin in mouse brain sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin using MBS600695. The blue is Nissl stain (rough ER), the red is nestin (MBS600695), and the green is either lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes). Photo courtesy of Robert Wine, NIEHS.)
Testing Data
(Immunolocalization of Nestin in mouse brain sections (hippocampus, CA3 region) from 21 day old mice 72 hrs after dosing with trimethyltin using MBS600695. The blue is Nissl stain (rough ER), the red is nestin (MBS600695), and the green is either lectin IB4 (microglia) or GFAP (glial fibrillary acidic protein, astrocytes). Photo courtesy of Robert Wine, NIEHS.)
NCBI and Uniprot Product Information
NCBI Description
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
Uniprot Description
nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 1q23.1
Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament
Molecular Function: intermediate filament binding; structural molecule activity
Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; brain development; negative regulation of neuron apoptosis; cell projection morphogenesis; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development
Research Articles on NES
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Product Notes
The NES nes (Catalog #AAA600695) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nestin (ESTM46, FLJ21841, Intermediate Filament Protein, Nbla00170, Nes) reacts with Mouse, Rat, Rodent and may cross-react with other species as described in the data sheet. AAA Biotech's Nestin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Dilution: Immunohistochemistry (Frozen or paraffin): 1:20-1:200. Perfuse tissue with 4% paraformaldehyde, pH 7.4 for light microscopy or with either 4% paraformaldehyde, pH 10 or 4% paraformaldehyde with 0.1% glutaraldehyde, pH 7.4 for EM. Western Blot: 200-220kD in reducing gels of newborn rat or mouse cell extracts. Prepare samples by boiling in 4 volumes of 125mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol, 4.6% SDS. 5% PAGE gels are suggested. Block membranes with milk or BSA. Do not dilute primary antibody in buffer containing milk. Freezing and thawing of samples or samples not stored at -70 degree C will show breakdown of nestin sometimes into multiple fragments. Immunocytochemistry: 1:20-1:200. Fix cells in 4% paraformaldehyde buffered with 50mM sodium borate, pH 9.5. Researchers should empirically determine the suitability of the NES nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nestin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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