Immunoblotting (IB)
(Figure 1 Immunoblotting of U251-cell lysate showing reactivity with nestin)
Mouse anti-Human Nestin Monoclonal Antibody | anti-NES antibody
Mouse anti Nestin
Gene Names
NES; FLJ21841; Nbla00170
Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
Nestin; Monoclonal Antibody; Mouse anti Nestin; anti-NES antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10C2
Specificity
RNF406 is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues.
Form/Format
Each vial contains 1 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-NES antibody
Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
10C2 Nestin is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, and Western blot. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 to 1:200 for immunocytochemistry and immunohistochemistry with a fluorescein or rhodamine conjugated anti-Mouse secondary antibody, and 1:1000 to 1:5000 for Western blot appliCations using chemoluminescent detection.
Source Note
Nestin (clone 10C2) is a monoclonal antibody derived by traditional hybridoma technology. A 150-amino-acid fragment from the cloned Human nestin was conjugated to glutathione S-transferase and used as an immunogen.
Preparation and Storage
Can be stored at -20 degree C to -70 degree C for long term storage. Once thawed, store at 4 degree C, do not refreeze.
Immunoblotting (IB)
(Figure 1 Immunoblotting of U251-cell lysate showing reactivity with nestin)
Immunoblotting (IB)
(Figure 1 Immunoblotting of U251-cell lysate showing reactivity with nestin)
Testing Data
(Figure 2 Immunostaining of intracellular nestin filaments in U251 cells)
Testing Data
(Figure 2 Immunostaining of intracellular nestin filaments in U251 cells)
Testing Data
(Figure 3 Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells (1:100 dilution).)
Testing Data
(Figure 3 Immunostaining of intracellular nestin filaments in cultured SH-SY-5Y neuronal cells (1:100 dilution).)
Related Product Information for anti-NES antibody
Nestin is a Class IV intermediate filament protein abundantly produced in the developing central nervous system (CNS) and in early embryonic neuroepithelial stem cells. This cytoskeletal protein has been widely used as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Also, it is a superior angiogenic marker to evaluate neovascularity of endothelial cells in tumors. These highly specific antibodies to Human nestin can aid in characterizing progenitor cells differentiating into distinct lineages, in enhancing the therapeutic potential of Human neural stem/progenitor cells in the treatment of CNS diseases or injury, and in identifying neuroepithelial tumor cells.
Product Categories/Family for anti-NES antibody
References
Messam, C., Hou, J. and Major, E.O. (2000). Co-expression of nestin in neural and glial cells in the developing Human CNS defined by a Human specific anti-nestin antibody. Exp Neurol. 161, 585-96. Messam, C., Hou, J., Berman, J.W., and Major, E.O. (2002) Analysis of the temporal expression of nestin in Human fetal brain derived neuronal and glial progenitor cells. Brain Res Dev Brain Res. 134, 87-92. Hou, Y.Y., et al. (2006). Schwannoma of the gastrointestinal tract: a clinicopathological, immunohistochemical and ultrastructural study of 33 cases. Histopathology 48, 536-45. Mokry, J., Pudil, R., Ehrmann, J., Cizkova, D., Osterreicher, J., Filip, S. and Kolar, Z. (2008). Re-expression of nestin in the myocardium of postinfarcted patients. Virchows Arch 453, 33-41. Corsino, P.E., et al. (2008). Mammary tumors initiated by constitutive Cdk2 activation contain an invasive basal-like component. Neoplasia 10, 1240-52. Murphy, A.J., Viero, S., Ho, M. and Thorner, P.S. (2009). Diagnostic utility of nestin expression in pediatric tumors in the region of the kidney. Appl Immunohistochem Mol Morphol. 17, 517-23. Sellheyer, K. and Krahl, D. (2010). Spatiotemporal expression pattern of neuroepithelial stem cell marker nestin suggests a role in dermal homeostasis, neovasculogenesis, and tumor stroma development: a study on embryonic and adult Human skin. J. Am. Acad. Dermatol. 63, 93-113. Elamin, M.H., et al. (2010). Curcumin inhibits the Sonic Hedgehog signaling pathway and triggers apoptosis in medulloblastoma cells. Mol. Carcinog. 49, 302-14. Popescu, L.M., et al. (2010). Telocytes in Human epicardium. J. Cell. Mol. Med. 14, 2085-93. Sellheyer, K. and Krahl, D. (2011). Does the peritumoral stroma of basal cell carcinoma recapitulate the follicular connective tissue sheath? J. Cutan. Pathol. 38, 551-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
177,439 Da
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
NES
NCBI Official Synonym Symbols
FLJ21841; Nbla00170
NCBI Protein Information
nestin; OTTHUMP00000032198
UniProt Protein Name
Nestin
Protein Family
UniProt Gene Name
NES
UniProt Entry Name
NEST_HUMAN
NCBI Description
Nestin is an intermediate filament protein that was first identified with a monoclonal antibody by Hockfield and McKay (1985) [PubMed 4078630]. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.[supplied by OMIM]
Uniprot Description
nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 1q23.1
Cellular Component: intermediate filament cytoskeleton; cytoplasm; intermediate filament
Molecular Function: intermediate filament binding; structural molecule activity
Biological Process: positive regulation of intermediate filament depolymerization; central nervous system development; negative regulation of catalytic activity; negative regulation of neuron apoptosis; brain development; cell projection morphogenesis; negative regulation of protein binding; G2/M transition of mitotic cell cycle; embryonic camera-type eye development
Research Articles on NES
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Product Notes
The NES nes (Catalog #AAA570163) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Nestin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Nestin can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (IHC), Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). 10C2 Nestin is useful for immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues, and Western blot. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 to 1:200 for immunocytochemistry and immunohistochemistry with a fluorescein or rhodamine conjugated anti-Mouse secondary antibody, and 1:1000 to 1:5000 for Western blot appliCations using chemoluminescent detection. Researchers should empirically determine the suitability of the NES nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nestin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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