Western Blot (WB)
(Western blot analysis of extracts of rat braincell lines, using NES antibody.)
Rabbit Nestin Antibody | anti-NES antibody
Nestin Antibody
Gene Names
NES; Nbla00170
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence
Purity
Affinity purification
Synonyms
Nestin; Nestin Antibody; FLJ21841; Nbla00170; anti-NES antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
1621
Applicable Applications for anti-NES antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:1000
IF: 1:20-1:50
IF: 1:20-1:50
Immunogen
A synthetic peptide of human NES
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of rat braincell lines, using NES antibody.)
Western Blot (WB)
(Western blot analysis of extracts of rat braincell lines, using NES antibody.)
Related Product Information for anti-NES antibody
Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1, 2). Upon maturation of the brain, nestin expression is quickly down-regulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, astrocytomas, and is generally accepted as a marker for neural cancer stem cells (3). Nestin expression has alos been observed in astrocytes, retina, cardiac muscle, pancreas and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.
Product Categories/Family for anti-NES antibody
References
1. Michalczyk, K. and Ziman, M. (2005) Histol Histopathol 20, 665-71. 2. Giliarov, A.V. (2007) Morfologiia 131, 85-90. 3. Gilyarov, A.V. (2008) Neurosci Behav Physiol 38, 165-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
177kDa
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
NES
NCBI Official Synonym Symbols
Nbla00170
NCBI Protein Information
nestin
UniProt Protein Name
Nestin
Protein Family
UniProt Gene Name
NES
NCBI Description
This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]
Uniprot Description
Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells ().
Research Articles on NES
Similar Products
Product Notes
The NES nes (Catalog #AAA8500061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Nestin Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nestin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF). WB: 1:500-1:1000 IF: 1:20-1:50. Researchers should empirically determine the suitability of the NES nes for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nestin, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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