Human Nestin ELISA Kit | NES elisa kit

Human Nestin ELISA Kit

Cat. Num. AAA760286

• Gene Names: NES; Nbla00170
• Reactivity: Human
• Synonyms: Nestin; Human Nestin ELISA Kit; NES/FLJ21841/nestin; NES elisa kit

• Reactivity: Human
• Sequence Length: 1621

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 15.625-1000pg/ml
• Sensitivity: 9.375pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for NES elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human NES antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human NES antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human NES amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human NES can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 38176300
• NCBI GeneID: 10763
• NCBI Accession #: NP_006608.1
• NCBI GenBank Nucleotide #: NM_006617.1
• Molecular Weight: 177,439 Da
• NCBI Official Full Name: nestin
• NCBI Official Synonym Full Names: nestin
• NCBI Official Symbol: NES
• NCBI Official Synonym Symbols: Nbla00170
• NCBI Protein Information: nestin
• UniProt Protein Name: Nestin
• Protein Family: Nestin
• UniProt Gene Name: NES
• UniProt Entry Name: NEST_HUMAN

NCBI Description

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

Uniprot Description

nestin: an intermediate filament protein. It is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon terminal neural differentiation, nestin is downregulated and replaced by neurofilaments.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 1q23.1

Cellular Component: cytoplasm; intermediate filament; intermediate filament cytoskeleton

Molecular Function: intermediate filament binding; structural molecule activity

Biological Process: brain development; cell projection morphogenesis; central nervous system development; embryonic camera-type eye development; G2/M transition of mitotic cell cycle; negative regulation of catalytic activity; negative regulation of neuron apoptosis; negative regulation of protein binding; positive regulation of intermediate filament depolymerization