Western Blot (WB)
(Anti-AHR antibody, MBS176885, Western blottingLane 1: Mouse Brain Tissue LysateLane 2: Mouse Heart Tissue LysateLane 3: Mouse Liver Tissue Lysate )
Rabbit anti-Mouse Aryl hydrocarbon Receptor Polyclonal Antibody | anti-AHR antibody
Anti-Aryl hydrocarbon Receptor antibody
IHC-P: (Concentration) 0.5-1ug/ml; (Antigen Retrieval) By Heat
Tested Species: In-house tested species with positive test results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin ssections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal diluions should be determined by end users.
Western Blot (WB)
(Anti-AHR antibody, MBS176885, Western blottingLane 1: Mouse Brain Tissue LysateLane 2: Mouse Heart Tissue LysateLane 3: Mouse Liver Tissue Lysate )
Western Blot (WB)
(Anti-AHR antibody, MBS176885, Western blottingLane 1: Mouse Brain Tissue LysateLane 2: Mouse Heart Tissue LysateLane 3: Mouse Liver Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-AHR antibody, MBS176885, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue )
Immunohistochemistry (IHC)
(Anti-AHR antibody, MBS176885, IHC(P)IHC(P): Mouse Cardiac Muscle Tissue )
Immunohistochemistry (IHC)
(Anti-AHR antibody, MBS176885, IHC(P)IHC(P): Mouse Brain Tissue )
Immunohistochemistry (IHC)
(Anti-AHR antibody, MBS176885, IHC(P)IHC(P): Mouse Brain Tissue )
Background: AHR(aryl hydrocarbon receptor), also called bHLHe76, is a member of the family of basic helix-loop-helix transcription factors. AhR is a cytosolic transcription factor that is normally inactive, bound to several co-chaperones. The AHR gene is mapped on 7p21.1. Estrogenic actions of AHR agonists were detected in wildtype ovariectomized mouse uteri, but were absent in Ahr -/- or Er-alpha -/- ovariectomized mice. Complex assembly and ubiquitin ligase activity of CUL4B(AHR) in vitro and in vivo are dependent on the AHR ligand. In the CUL4B(AHR) complex, ligand-activated AHR acts as a substrate-specific adaptor component that targets sex steroid receptors for degradation. Cd4-positive cells from mice lacking Ahr developed Th17 responses but failed to produce Il22 and did not show enhanced Th17 development. Activation of Ahr during induction of EAE accelerated disease onset and increased pathology in wildtype mice, but not in Ahr -/- mice. The TDO-AHR pathway is active in human brain tumors and is associated with malignant progression and poor survival. Ahr activity within ROR-gamma-t-positive ILC could be induced by dietary ligands such as those contained in vegetables of the family Brassicaceae.
NCBI and Uniprot Product Information
Uniprot Description
AHR: a nuclear receptor for aryl hydrocarbons involved in xenobiotic metabolism, cell cycle regulation, and development in response to both endogenous and environmental signals. AhR was initially identified as a receptor for dioxins, which are environmental pollutants generated by waste incineration and other industrial processes . AhR ligands include polycyclic aromatic hydrocarbons, including the carcinogen benzo(a)pyrene and other components of cigarette smoke. Naturally occurring AhR ligands include flavonoids, which are aromatic plant secondary compounds commonly found in vegetables and fruits. Cytoplasmic aryl hydrocarbon receptors are found in protein complexes with heat shock proteins. Upon ligand binding, AhR dissociates from heat shock proteins and translocate to the nucleus where it dimerizes with AhR nuclear translocator (ARNT, HIF-1b). The AhR/ARNT heterodimer binds to nuclear xenobiotic response elements to control the expression of genes associated with xenobiotic metabolism, including several cytochrome P450 genes. AhR is ubiquitously expressed and is thought to play a role in regulation of cell proliferation and differentiation, cytokine expression, and xenobiotic metabolism. Research studies link AhR activity with the control of regulatory T-cell and T-helper 17 cell differentiation, regulation of the inflammatory response, and the onset of lung cancer.
Protein type: Transcription factor; DNA-binding; Nuclear receptor
Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus; cytosol
Molecular Function: protein dimerization activity; RNA polymerase II transcription factor activity, enhancer binding; protein binding; signal transducer activity; ligand-dependent nuclear receptor activity; DNA binding; protein heterodimerization activity; sequence-specific DNA binding; aryl hydrocarbon receptor activity; Hsp90 protein binding; transcription factor binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; prostate gland development; gland development; positive regulation of cell size; blood circulation; response to toxin; positive regulation of transcription, DNA-dependent; rhythmic process; cell morphogenesis; negative regulation of transcription from RNA polymerase II promoter; signal transduction; response to organic cyclic substance; smooth muscle development; ovarian follicle development; B cell homeostasis; regulation of transcription, DNA-dependent; post-embryonic hemopoiesis; regulation of blood pressure; response to stress; T cell homeostasis; regulation of B cell proliferation; circadian regulation of gene expression; regulation of blood vessel size; positive regulation of transcriptional preinitiation complex assembly; positive regulation of growth; spleen development; blood vessel development; camera-type eye development; intracellular receptor-mediated signaling pathway; transcription, DNA-dependent; immune system process; embryonic hemopoiesis; negative regulation of systemic arterial blood pressure; B-1 B cell homeostasis; liver development; cell cycle; negative regulation of vasoconstriction; regulation of transcription from RNA polymerase II promoter; patterning of blood vessels; response to xenobiotic stimulus; regulation of gene expression; B cell differentiation; xenobiotic metabolic process; blood vessel morphogenesis; blood vessel remodeling; negative regulation of transcription, DNA-dependent; reproductive structure development; lymphocyte homeostasis
Research Articles on AHR
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Product Notes
The AHR ahr (Catalog #AAA176885) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Aryl hydrocarbon Receptor antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Aryl hydrocarbon Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. WB: (Concentration) 0.1-0.5ug/ml IHC-P: (Concentration) 0.5-1ug/ml; (Antigen Retrieval) By Heat Tested Species: In-house tested species with positive test results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin ssections in 10mM citrate buffer, pH6.0, for 20 mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal diluions should be determined by end users. Researchers should empirically determine the suitability of the AHR ahr for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Aryl hydrocarbon Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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