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Western Blot (WB) (Western blot analysis MRPL53 using HeLa whole cell lysates)

Rabbit MRPL53 Polyclonal Antibody | anti-MRPL53 antibody

MRPL53 Antibody

Gene Names
MRPL53; L53MT
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Synonyms
MRPL53; Polyclonal Antibody; MRPL53 Antibody; 39S ribosomal protein L53; mitochondrial; anti-MRPL53 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
MRPL53 Antibody detects endogenous levels of total MRPL53
Form/Format
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (varies by lot)
Sequence Length
112
Applicable Applications for anti-MRPL53 antibody
Western Blot (WB)
Application Notes
Western Blot: 1:1000-3000
Immunogen
A synthesized peptide derived from human MRPL53
Immunogen Type
Peptide
Preparation and Storage
Store at -20 degree C

Western Blot (WB)

(Western blot analysis MRPL53 using HeLa whole cell lysates)

Western Blot (WB) (Western blot analysis MRPL53 using HeLa whole cell lysates)
Product Categories/Family for anti-MRPL53 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,107 Da
NCBI Official Full Name
39S ribosomal protein L53, mitochondrial
NCBI Official Synonym Full Names
mitochondrial ribosomal protein L53
NCBI Official Symbol
MRPL53
NCBI Official Synonym Symbols
L53MT
NCBI Protein Information
39S ribosomal protein L53, mitochondrial
UniProt Protein Name
39S ribosomal protein L53, mitochondrial
Protein Family
UniProt Gene Name
MRPL53
UniProt Synonym Gene Names
L53mt; MRP-L53
UniProt Entry Name
RM53_HUMAN

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 1p. [provided by RefSeq, Jul 2008]

Uniprot Description

MRPL53: Component of the mitochondrial ribosome large subunit (39S) which comprises a 16S rRNA and about 50 distinct proteins. {ECO:0000269|PubMed:25278503, ECO:0000269|PubMed:25838379}

Protein type: Mitochondrial; Ribosomal

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: mitochondrial inner membrane; mitochondrial large ribosomal subunit

Molecular Function: protein binding

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Product Notes

The MRPL53 mrpl53 (Catalog #AAA9419116) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MRPL53 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's MRPL53 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: 1:1000-3000. Researchers should empirically determine the suitability of the MRPL53 mrpl53 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MRPL53, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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