Rat JAM-C ELISA Kit | JAM-C elisa kit

Rat JAM-C PicoKine ELISA Kit

Cat. Num. AAA1751654

• Gene Names: JAM3; JAMC; JAM-2; JAM-3; JAM-C
• Reactivity: Rat
• Synonyms: JAM-C; Rat JAM-C PicoKine ELISA Kit; Rat JAM-C; JAM-C elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant Rat Il6st
• Sequence Length: 259

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
• Detection Range: 0.78ng/ml-50ng/ml
• Sensitivity: <0.05ng/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for JAM-C elisa kit

Principle of the Assay: The Picokine Rat Il6st Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Rat Il6st with a 96-well strip plate that is pre-coated with antibody specific for Il6st. The detection antibody is a biotinylated antibody specific for Il6st. The capture antibody is A monoclonal antibody from mouse, the detection antibody is a biotinylated detection polyclonal antibody from goat. The kit contains recombinant Rat Il6st with immunogen: Expression system for standard: NSO, Immunogen sequence: Q23-E618. The kit is analytically validated with ready to use reagents. To measure Rat Il6st, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Rat Il6st in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Rat Il6st in the sample!!Background/Introduction: GP130(Glycoprotein 130) also known as IL6ST or CD130, is a transmembrane protein which is the founding member of the class of all cytokine receptors. The GP130 gene is mapped on 5q11.2. Gp130 showed an apparent molecular mass of 130 kD by SDS-PAGE. Gp130 is involved in the formation of high-affinity IL6-binding sites and in IL6 signal transduction. Sgp130 has a role in modulating signals transduced by membranebound gp130. Gp130 is an important part of many different types of signaling complexes. Gp130 molecules with mutations in either of these segments could not transduce a growth signal.

NCBI and Uniprot Product Information

• NCBI GI #: 328927057
• NCBI GeneID: 83700
• NCBI Accession #: NP_001192258.1
• NCBI GenBank Nucleotide #: NM_001205329.1
• NCBI Official Full Name: junctional adhesion molecule C isoform 2
• NCBI Official Synonym Full Names: junctional adhesion molecule 3
• NCBI Official Symbol: JAM3
• NCBI Official Synonym Symbols: JAMC; JAM-2; JAM-3; JAM-C
• NCBI Protein Information: junctional adhesion molecule C

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]