Western Blot (WB)
(Western blot analysis of MEK1: Lane 1: MW marker, Lane 2: GST-tagged recombinant MEK1)
Mouse anti-Human MEK1 Monoclonal Antibody | anti-MKK1; MAP kinase kinase 1 antibody
MEK1 monoclonal antibody (3D9)
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Protein G affinity purified.
Synonyms
MEK1; Monoclonal Antibody; MEK1 monoclonal antibody (3D9); anti-MKK1; MAP kinase kinase 1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3D9
Specificity
Recognizes human MEK1. Detects a band of ~45kDa (or ~71kDa with GST tag) by Western blot.
Purity/Purification
Protein G affinity purified.
Form/Format
Liquid. In PBS containing 0.1% sodium azide. Concentration 1mg/ml.
Sequence Length
354
Applicable Applications for anti-MKK1; MAP kinase kinase 1 antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
Immunohistochemistry (1ug/ml)
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~45kDa (or ~71kDa with GST tag) by Western blot.
Suggested dilutions/conditions may not be available for all applications.
Detects a band of ~45kDa (or ~71kDa with GST tag) by Western blot.
Immunogen
Native MEK and recombinant MEK1.
Preparation and Storage
Long term storage: -20 degree C
Avoid freeze/thaw cycles.
Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of MEK1: Lane 1: MW marker, Lane 2: GST-tagged recombinant MEK1)
Western Blot (WB)
(Western blot analysis of MEK1: Lane 1: MW marker, Lane 2: GST-tagged recombinant MEK1)
Related Product Information for anti-MKK1; MAP kinase kinase 1 antibody
MEK is a dual specificity kinase capable of phosphorylating both tyrosine and threonine residues. The MEK family, which is also known as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif; phosphorylation of MAPK by MEK results in an increase in MAPK activity. MEK is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.
Product Categories/Family for anti-MKK1; MAP kinase kinase 1 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
34,087 Da
NCBI Official Full Name
MEK1
UniProt Protein Name
Mitogen-activated protein kinase kinase 1
UniProt Gene Name
MKK1
UniProt Synonym Gene Names
MEK1; AtMKK1; MAP kinase kinase 1
UniProt Entry Name
M2K1_ARATH
Uniprot Description
MEKK1, MKK1/MKK2 and MPK4/MPK6 function in a signaling pathway that modulates the expression of genes responding to biotic and abiotic stresses and also plays an important role in pathogen defense by negatively regulating innate immunity. Activates by phosphorylation the downstream MPK4. Acts redundantly with MKK2. MKK1-MPK6 module mediates abscisic acid (ABA)-dependent CAT1 expression with H2O2 production and response to drought and salt stress. MKK1-MPK6 module is also involved in sugar signaling during the process of seed germination.
Similar Products
Product Notes
The MKK1; MAP kinase kinase 1 mkk1 (Catalog #AAA565093) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MEK1 monoclonal antibody (3D9) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). Immunohistochemistry (1ug/ml) Suggested dilutions/conditions may not be available for all applications. Detects a band of ~45kDa (or ~71kDa with GST tag) by Western blot. Researchers should empirically determine the suitability of the MKK1; MAP kinase kinase 1 mkk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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