Rabbit MEK1 Polyclonal Antibody | anti-MKK1; MAP kinase kinase 1 antibody

MEK1 polyclonal antibody

Cat. Num. AAA565274

• Gene Names: MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• Reactivity: Human, Mouse, Rat, Xenopus
• Applications: Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Protein A affinity purified.
• Synonyms: MEK1; Polyclonal Antibody; MEK1 polyclonal antibody; anti-MKK1; MAP kinase kinase 1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Xenopus
• Clonality: Polyclonal
• Specificity: Recognizes human, mouse, rat and Xenopus MEK1. Detect a band of ~48kDa by Western blot.
• Purity/Purification: Protein A affinity purified.
• Form/Format: Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.
• Sequence Length: 393

• Applicable Applications for anti-MKK1; MAP kinase kinase 1 antibody: Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Immunohistochemistry (1:50); Western Blot (1ug/ml, colorimetric); Suggested dilutions/conditions may not be available for all applications.; Detects a band of ~48kDa by Western blot.
• Immunogen: Synthetic peptide corresponding to the sequence near the N-terminus of human MEK1. The sequence is completely conserved in mouse, rat, hamster, rabbit and Xenopus.
• Preparation and Storage: Long term storage: -20 degree C; Avoid freeze/thaw cycles.

Immunofluorescence (IF)

(Immunofluorescent analysis (confocal) staining of HeLa cells using MEK1 pAb (green); nuclei are stained in blue pseudocolor using DRAQ5.)

Related Product Information for anti-MKK1; MAP kinase kinase 1 antibody

MEK is a dual specificity kinase capable of phosphorylating both tyrosine and threonine residues. The MEK family, which is also known as MAP kinase kinase, phosphorylates MAP kinases on the conserved T-X-Y motif; phosphorylation of MAPK by MEK results in an increase in MAPK activity. MEK is involved in a diverse array of cellular processes such as stress-activated response, apoptosis, cytokine-induced cell proliferation, and DNA recombination during meiosis.

Product Categories/Family for anti-MKK1; MAP kinase kinase 1 antibody

Posttranslational Modifications; Cell Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 400274
• NCBI GeneID: 5604
• UniProt Accession #: Q02750
• Molecular Weight: 40,764 Da
• NCBI Official Full Name: Dual specificity mitogen-activated protein kinase kinase 1
• NCBI Official Synonym Full Names: mitogen-activated protein kinase kinase 1
• NCBI Official Symbol: MAP2K1
• NCBI Official Synonym Symbols: CFC3; MEK1; MKK1; MAPKK1; PRKMK1
• NCBI Protein Information: dual specificity mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Dual specificity mitogen-activated protein kinase kinase 1
• UniProt Gene Name: MAP2K1
• UniProt Synonym Gene Names: MEK1; PRKMK1; MAP kinase kinase 1; MAPKK 1; MKK1; MEK 1
• UniProt Entry Name: MP2K1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]

Uniprot Description

MEK1: a dual-specificity protein kinase of the STE7 kinase family. Phosphorylated and activated by Raf, Mos and Cot kinases. Phosphorylates a Thr and a Tyr residue in a Thr-Glu-Tyr sequence located in the activation loop of ERK1 and ERK2. An essential component of MAP kinase signal transduction pathways involved in many cellular processes such as proliferation, differentiation, transcription regulation and development.

Protein type: Protein kinase, dual-specificity (non-receptor); Kinase, protein; Protein kinase, STE; EC 2.7.12.2; STE group; STE7 family

Chromosomal Location of Human Ortholog: 15q22.1-q22.33

Cellular Component: cytoplasm; cytosol; early endosome; endoplasmic reticulum; focal adhesion; Golgi apparatus; late endosome; microtubule organizing center; mitochondrion; nucleus; plasma membrane

Molecular Function: ATP binding; MAP kinase kinase activity; protein binding; protein C-terminus binding; protein kinase activity; protein N-terminus binding; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; protein-tyrosine kinase activity; receptor signaling protein tyrosine phosphatase activity

Biological Process: activation of MAPK activity; activation of MAPKK activity; axon guidance; Bergmann glial cell differentiation; cell cycle arrest; cell motility; cell motility involved in cell locomotion; cerebellar cortex formation; chemotaxis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; heart development; innate immune response; insulin receptor signaling pathway; keratinocyte differentiation; MAPKKK cascade; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of cell proliferation; nerve growth factor receptor signaling pathway; peptidyl-tyrosine phosphorylation; positive regulation of axonogenesis; Ras protein signal transduction; regulation of apoptosis; regulation of axon regeneration; regulation of mitotic cell cycle; regulation of stress-activated MAPK cascade; signal transduction; small GTPase mediated signal transduction; stress-activated MAPK cascade; stress-activated protein kinase signaling pathway; thymus development; thyroid gland development; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway

Disease: Cardiofaciocutaneous Syndrome 3; Noonan Syndrome 1