Mouse Internexin, alpha Monoclonal Antibody | anti-INA antibody

Internexin, alpha (alpha-Internexin, alpha-Inx, INA, Inexa, 66 kD Neurofilament Protein, Neurofilament-66, NF66, NF-66, Neurofilament 5, NEF5)

Cat. Num. AAA643502

• Gene Names: INA; NEF5; NF-66; TXBP-1
• Reactivity: Feline, Human, Mammal, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: Internexin; alpha; Monoclonal Antibody; alpha (alpha-Internexin; alpha-Inx; INA; Inexa; 66 kD Neurofilament Protein; Neurofilament-66; NF66; NF-66; Neurofilament 5; NEF5); Anti -Internexin; anti-INA antibody

• Host: Mouse
• Reactivity: Feline, Human, Mammal, Rat
• Clonality: Monoclonal
• Clone Number: 13B826
• Specificity: Recognizes human a-Internexin and with this protein in all mammalian species tested to date.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-INA antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot.; Dilution: Immunocytochemistry (ABC): 1:5000; Immunofluorescence: 1:500; Western Blot: 1:10,000
• Immunogen: Purified recombinant rat a-internexin expressed in and purified from E. coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Left three lanes show Coomassie brilliant blue stained extracts of rat brain stem, cat cerebral cortex and human cerebral cortex (R, C and H respectively). Right three lanes are corresponding blots reacted with MBS643502. MBS643502 stains a band of about 66kD in all three preparations.)

Testing Data

(Mixed cultures of rat CNS cells stained with MBS643502 (red) and chicken antibody to Microtubule associated protein 2 (MAP2- green, using antibody 031388). The a-internexin is localized primarily in neuronal axons in these cultures, while the perikarya and dendrites of neurons stain strongly for MAP2.)

Related Product Information for anti-INA antibody

a-Internexin is a Class IV intermediate filament subunit. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H, and a-internexin is the major neurofilament protein of developing neurons. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient feature of neuronal progenitors cells and other cell committed to the neuronal lineage. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. The HGNC name for this protein is INA.

Product Categories/Family for anti-INA antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 119570039
• NCBI GeneID: 9118
• UniProt Accession #: Q16352; Q9BRC5; B1AQK0
• Molecular Weight: 55,391 Da
• NCBI Official Full Name: internexin neuronal intermediate filament protein, alpha, isoform CRA_b
• NCBI Official Synonym Full Names: internexin neuronal intermediate filament protein, alpha
• NCBI Official Symbol: INA
• NCBI Official Synonym Symbols: NEF5; NF-66; TXBP-1
• NCBI Protein Information: alpha-internexin; alpha-Inx; neurofilament 5 (66kD); 66 kDa neurofilament protein; neurofilament-66, tax-binding protein
• UniProt Protein Name: Alpha-internexin
• Protein Family: Immune inhibitor
• UniProt Gene Name: INA
• UniProt Synonym Gene Names: NEF5; Alpha-Inx; NF-66
• UniProt Entry Name: AINX_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]

Uniprot Description

INA: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; extracellular space; intermediate filament cytoskeleton; nuclear membrane; neurofilament

Molecular Function: structural constituent of cytoskeleton

Biological Process: substantia nigra development; neurofilament cytoskeleton organization and biogenesis; cell differentiation