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Typical Testing Data/Standard Curve (for reference only)

Nuclear respiratory factor 1, NRF1 ELISA Kit | NRF1 elisa kit

Rat Nuclear respiratory factor 1, NRF1 ELISA Kit

Gene Names
NRF1; ALPHA-PAL
Reactivity
Rat
Synonyms
Nuclear respiratory factor 1; NRF1; Rat Nuclear respiratory factor 1; NRF1 ELISA Kit; NRF1 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Sequence Length
522
Samples
Serum, plasma, cell culture supernates, cell lysates, tissue homogenates
Assay Type
Quantitative Sandwich
Detection Range
0.2ng/ml - 60ng/ml
Sensitivity
0.11ng/ml
Intra-Assay
CV<8%
Inter-Assay
CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for NRF1 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of Rat Nuclear Respiratory Factor 1 (also known as NRF1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat NRF1 antibody. NRF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NRF1 Antibody is added and binds to NRF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NRF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NRF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
Molecular Weight
46,157 Da
NCBI Official Full Name
NRF1 protein
NCBI Official Synonym Full Names
nuclear respiratory factor 1
NCBI Official Symbol
NRF1
NCBI Official Synonym Symbols
ALPHA-PAL
NCBI Protein Information
nuclear respiratory factor 1
UniProt Protein Name
Nuclear respiratory factor 1
UniProt Gene Name
NRF1
UniProt Synonym Gene Names
NRF-1; Alpha-pal

NCBI Description

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]

Uniprot Description

Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.

Research Articles on NRF1

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Product Notes

The Rat NRF1 nrf1 (Catalog #AAA1600077) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1600077 ELISA Kit recognizes Rat NRF1. It is sometimes possible for the material contained within the vial of "Nuclear respiratory factor 1, NRF1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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