Nuclear respiratory factor 1, NRF1 ELISA Kit | NRF1 elisa kit
Rat Nuclear respiratory factor 1, NRF1 ELISA Kit
Principles of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Rat NRF1 antibody. NRF1 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Rat NRF1 Antibody is added and binds to NRF1 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated NRF1 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Rat NRF1. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]
Uniprot Description
Transcription factor that activates the expression of the EIF2S1 (EIF2-alpha) gene. Links the transcriptional modulation of key metabolic genes to cellular growth and development. Implicated in the control of nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication.
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Product Notes
The Rat NRF1 nrf1 (Catalog #AAA1600077) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1600077 ELISA Kit recognizes Rat NRF1. It is sometimes possible for the material contained within the vial of "Nuclear respiratory factor 1, NRF1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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