Mouse anti-Human, Rat Internexin, alpha Monoclonal Antibody | anti-INA antibody

Internexin, alpha (alpha-Internexin, alpha-Inx, INA, Inexa, 66 kD Neurofilament Protein, Neurofilament-66, NF66, NF-66, Neurofilament 5, NEF5)

Cat. Num. AAA6008330

• Gene Names: INA; NEF5; NF-66; TXBP-1
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: Internexin; alpha; Monoclonal Antibody; alpha (alpha-Internexin; alpha-Inx; INA; Inexa; 66 kD Neurofilament Protein; Neurofilament-66; NF66; NF-66; Neurofilament 5; NEF5); Anti -Internexin; anti-INA antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Clone Number: 13B827
• Specificity: Recognizes human a-Internexin and with this protein in all mammalian species tested to date.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-INA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Immunohistochemistry and Western Blot.; Dilution: Immunofluorescence: 1:500; Immunohistochemistry (ABC): 1:5000; Western Blot: 1:10,000
• Immunogen: Recombinant rat a-Internexin expressed in and purified from E. coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Blot of homogenate of rat facial nucleus incubated with MBS6008330, which reveals a single clean band running at ~66kD.)

Testing Data

(Rat facial nucleus before (left) and after (right) facial nerve lesion. Note the profound upregulation of a-internexin in the facial nucleus motor neurons.)

Related Product Information for anti-INA antibody

a-Internexin is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits (1). On SDS-PAGE gels it runs with an apparent molecular weight of 64 to 66kD, with some species variability, although the real molecular weight is about 55kD; as with the other neurofilament subunits the presence of highly negatively charged sequences results in reduction of SDS-PAGE mobility. a-internexin is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed only in neurons and in large amounts early in neuronal development, but is down-regulated in many neurons as development procedes. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of a-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. Recently Trojanowski, Lee and coworkers used this antibody to show that a-internexin is an abundant component of the inclusions of neurofilament inclusion body disease (NFID, see two Cairns et al. papers listed below), a serious human neurodegenerative disorder. The antibody was also used to confirm the presence of circulating auto-antibodies to a-internexin in the sera of some patients with endocrine autoimmunity, as well as in some normal individuals (Rajasalu et al., 2004, listed below). The HGNC name for this protein is INA.

Product Categories/Family for anti-INA antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 119570039
• NCBI GeneID: 9118
• UniProt Accession #: Q16352; Q9BRC5; B1AQK0
• Molecular Weight: 55,391 Da
• NCBI Official Full Name: internexin neuronal intermediate filament protein, alpha, isoform CRA_b
• NCBI Official Synonym Full Names: internexin neuronal intermediate filament protein, alpha
• NCBI Official Symbol: INA
• NCBI Official Synonym Symbols: NEF5; NF-66; TXBP-1
• NCBI Protein Information: alpha-internexin; alpha-Inx; neurofilament 5 (66kD); 66 kDa neurofilament protein; neurofilament-66, tax-binding protein
• UniProt Protein Name: Alpha-internexin
• Protein Family: Immune inhibitor
• UniProt Gene Name: INA
• UniProt Synonym Gene Names: NEF5; Alpha-Inx; NF-66
• UniProt Entry Name: AINX_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]

Uniprot Description

INA: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; extracellular space; intermediate filament cytoskeleton; nuclear membrane; neurofilament

Molecular Function: structural constituent of cytoskeleton

Biological Process: substantia nigra development; neurofilament cytoskeleton organization and biogenesis; cell differentiation