Rabbit anti-Rat Internexin, alpha Polyclonal Antibody | anti-INA antibody

Internexin, alpha (alpha-Internexin, alpha-Inx, Ina, Inexa, Neurofilament-66, NF66)

Cat. Num. AAA6008399

• Gene Names: INA; NEF5; NF-66; TXBP-1
• Reactivity: Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Serum; Serum
• Synonyms: Internexin; alpha; Polyclonal Antibody; alpha (alpha-Internexin; alpha-Inx; Ina; Inexa; Neurofilament-66; NF66); Anti -Internexin; anti-INA antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Recognizes rat a-Internexin.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid.

• Applicable Applications for anti-INA antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 1:500-1:1000; Western Blot: 1:10,000-1:20,000; On Western Blots look for a major band at 64 to 66kD, depending on the species, the human protein being a little larger than that from rodents.
• Immunogen: Recombinant alpha-internexin expressed in and purified from E. coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot of whole rat spinal cord homogenate stained with MBS6008399, at dilution of 1:20,000. A prominent band running at ~66kD is apparent, as well as smaller lower bands which are apparently degradation products. A minor band at ~150kD is also seen, apparently resulting from dimerization of alpha-internexin.)

Testing Data

(Mixed neuron-glial cultures stained with rabbit antibody to alpha-internexin (red) and chicken antibody to peripherin 031422 (green). The alpha internexin antibody stains numerous axonal and dendritic profiles in these cultures, while peripherin antibody binds to only a subset of neurons.)

Related Product Information for anti-INA antibody

Neurofilaments can be defined as the intermediate or 10nm diameter filaments found in neuronal cells. They are composed a mixture of subunits which often includes triplet proteins, NF-L, NF-M and NF-H. Neurofilaments may also include peripherin, alpha-internexin, nestin and in some cases vimentin. Alpha-internexin is a protein running on SDS-PAGE at ~64-66kD and is a Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits (1). It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to alpha-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of alpha-internexin during neuronal regeneration (2). The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest. The HGNC name for this protein is INA.

Product Categories/Family for anti-INA antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 119570039
• NCBI GeneID: 9118
• UniProt Accession #: Q16352; Q9BRC5; B1AQK0
• Molecular Weight: 55,391 Da
• NCBI Official Full Name: internexin neuronal intermediate filament protein, alpha, isoform CRA_b
• NCBI Official Synonym Full Names: internexin neuronal intermediate filament protein, alpha
• NCBI Official Symbol: INA
• NCBI Official Synonym Symbols: NEF5; NF-66; TXBP-1
• NCBI Protein Information: alpha-internexin; alpha-Inx; neurofilament 5 (66kD); 66 kDa neurofilament protein; neurofilament-66, tax-binding protein
• UniProt Protein Name: Alpha-internexin
• Protein Family: Immune inhibitor
• UniProt Gene Name: INA
• UniProt Synonym Gene Names: NEF5; Alpha-Inx; NF-66
• UniProt Entry Name: AINX_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]

Uniprot Description

INA: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; extracellular space; intermediate filament cytoskeleton; nuclear membrane; neurofilament

Molecular Function: structural constituent of cytoskeleton

Biological Process: substantia nigra development; neurofilament cytoskeleton organization and biogenesis; cell differentiation