Rabbit Internexin, alpha Polyclonal Antibody | anti-INA antibody

Internexin, alpha

Cat. Num. AAA619982

• Gene Names: INA; NEF5; NF-66; TXBP-1
• Reactivity: Human, Mammal, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Serum; Serum
• Synonyms: Internexin; alpha; Polyclonal Antibody; Anti -Internexin; anti-INA antibody

• Host: Rabbit
• Reactivity: Human, Mammal, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes a-internexin. Species Crossreactivity: Human and other mammalian samples.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS, pH7.6, 0.05% sodium azide, 0.01% thimerosal, 15mg/ml BSA before the addition of glycerol to 40%.

• Applicable Applications for anti-INA antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence.; Dilution: Immunohistochemistry: 1:500 Formalin-fixed paraffin-embedded histological sections, cryostat sections.; Western Blot: 1:2500. Detects a band at 66kD.; Immunofluorescence: 1:500
• Immunogen: Purified recombinant rat a-internexin
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(Alpha internexin (red, neuronal progenitor cells); green shows fibroblast marker (plectin) Photo courtesy of: Dr. Gerry Shaw University of Florida)

Related Product Information for anti-INA antibody

Alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Product Categories/Family for anti-INA antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 119570039
• NCBI GeneID: 9118
• UniProt Accession #: Q16352; Q9BRC5; B1AQK0
• Molecular Weight: 55,391 Da
• NCBI Official Full Name: internexin neuronal intermediate filament protein, alpha, isoform CRA_b
• NCBI Official Synonym Full Names: internexin neuronal intermediate filament protein, alpha
• NCBI Official Symbol: INA
• NCBI Official Synonym Symbols: NEF5; NF-66; TXBP-1
• NCBI Protein Information: alpha-internexin; alpha-Inx; neurofilament 5 (66kD); 66 kDa neurofilament protein; neurofilament-66, tax-binding protein
• UniProt Protein Name: Alpha-internexin
• Protein Family: Immune inhibitor
• UniProt Gene Name: INA
• UniProt Synonym Gene Names: NEF5; Alpha-Inx; NF-66
• UniProt Entry Name: AINX_HUMAN

NCBI Description

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene is a member of the intermediate filament family and is involved in the morphogenesis of neurons. [provided by RefSeq, Jun 2009]

Uniprot Description

INA: Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 10q24.33

Cellular Component: nucleoplasm; extracellular space; intermediate filament cytoskeleton; nuclear membrane; neurofilament

Molecular Function: structural constituent of cytoskeleton

Biological Process: substantia nigra development; neurofilament cytoskeleton organization and biogenesis; cell differentiation