Rabbit anti-Human, Mouse ADGRB1/BAI1 Polyclonal Antibody | anti-ADGRB1/BAI1 antibody

Anti-ADGRB1/BAI1 Antibody (aa683-732)

Cat. Num. AAA2401290

• Gene Names: ADGRB1; BAI1; GDAIF
• Reactivity: Human, Mouse
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: ADGRB1/BAI1; Polyclonal Antibody; Anti-ADGRB1/BAI1 Antibody (aa683-732); Rabbit Polyclonal to Human ADGRB1/BAI1; ADGRB1; BAI1; GDAIF; anti-ADGRB1/BAI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: BAI1 Antibodyantibody detects endogenous levels of BAI1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, pH 7.4, 150 mM sodium chloride, 0.02% sodium azide, 50% glycerol
• Sequence Length: 1584

• Applicable Applications for anti-ADGRB1/BAI1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: ELISA: 1:10000; IHC-P: 1:50; WB: 1:500-1:1000
• Immunogen: Synthetic peptide from human BAI1 (aa683-732).
• Immunogen Species: Human
• Epitope: aa683-732
• Target: ADGRB1/BAI1
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (WB)

(Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot of the lysates from HUVEC cells using BAI1 antibody.)

Related Product Information for anti-ADGRB1/BAI1 antibody

Angiogenesis is controlled by a local balance between stimulators and inhibitors of new vessel growth and is suppressed under normalphysiologic conditions. Angiogenesis has been shown to be essential for growth and metastasis of solid tumors. In order to obtain blood supplyfor their growth, tumor cells are potently angiogenic and attract new vessels as results of increased secretion of inducers and decreased production of endogenous negative regulators.

Product Categories/Family for anti-ADGRB1/BAI1 antibody

GPCR; Orphan-B

NCBI and Uniprot Product Information

• NCBI GI #: 111118994
• NCBI GeneID: 575
• NCBI Accession #: NP_001693.2
• NCBI GenBank Nucleotide #: NM_001702.2
• UniProt Accession #: O14514
• Molecular Weight: 173 kDa
• NCBI Official Full Name: adhesion G protein-coupled receptor B1
• NCBI Official Synonym Full Names: adhesion G protein-coupled receptor B1
• NCBI Official Symbol: ADGRB1
• NCBI Official Synonym Symbols: BAI1; GDAIF
• NCBI Protein Information: adhesion G protein-coupled receptor B1
• UniProt Protein Name: Adhesion G protein-coupled receptor B1
• UniProt Gene Name: ADGRB1
• UniProt Synonym Gene Names: Vstat120

NCBI Description

Angiogenesis is controlled by a local balance between stimulators and inhibitors of new vessel growth and is suppressed under normal physiologic conditions. Angiogenesis has been shown to be essential for growth and metastasis of solid tumors. In order to obtain blood supply for their growth, tumor cells are potently angiogenic and attract new vessels as results of increased secretion of inducers and decreased production of endogenous negative regulators. BAI1 contains at least one 'functional' p53-binding site within an intron, and its expression has been shown to be induced by wildtype p53. There are two other brain-specific angiogenesis inhibitor genes, designated BAI2 and BAI3 which along with BAI1 have similar tissue specificities and structures, however only BAI1 is transcriptionally regulated by p53. BAI1 is postulated to be a member of the secretin receptor family, an inhibitor of angiogenesis and a growth suppressor of glioblastomas [provided by RefSeq, Jul 2008]

Uniprot Description

Phosphatidylserine receptor which enhances the engulfment of apoptotic cells (PubMed:24509909). Also mediates the binding and engulfment of Gram-negative bacteria (PubMed:26838550). Stimulates production of reactive oxygen species by macrophages in response to Gram-negative bacteria, resulting in enhanced microbicidal macrophage activity (PubMed:26838550). In the gastric mucosa, required for recognition and engulfment of apoptotic gastric epithelial cells (PubMed:24509909). Promotes myoblast fusion (). Activates the Rho pathway in a G-protein-dependent manner (PubMed:23782696). Inhibits MDM2-mediated ubiquitination and degradation of DLG4/PSD95, promoting DLG4 stability and regulating synaptic plasticity (). Required for the formation of dendritic spines by ensuring the correct localization of PARD3 and TIAM1 (). Potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53/TP53 signal in suppression of glioblastoma (PubMed:11875720).