Mouse anti-Human c-Met Monoclonal Antibody | anti-MET antibody

Mouse Monoclonal (IgG2a) to Human c-Met

Cat. Num. AAA245381

• Gene Names: MET; HGFR; AUTS9; RCCP2; c-Met
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Purified
• Synonyms: c-Met; Monoclonal Antibody; Mouse Monoclonal (IgG2a) to Human c-Met; MET | AUTS9 | C-Met | HGF/SF receptor | HGFR | Met kinase | Proto-oncogene c-Met | Scatter factor receptor | SF receptor | HGF receptor | RCCP2 | Tyrosine-protein kinase Met; anti-MET antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Specificity: Recognizes c-Met.
• Purity/Purification: Purified
• Form/Format: PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
• Concentration: 1.14 mg/ml (varies by lot)
• Sequence Length: 1390

• Applicable Applications for anti-MET antibody: Immunohistochemistry (IHC - Paraffin; IHC - Frozen)
• Application Notes: IHC - Paraffin: (10 µg/ml); IHC - Frozen (1:50); Immunohistochemistry: MBS245381 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245381 was determined to be 10 ug/ml. Positive control: Breast cancer.; ; (Optimal dilution to be determined by the researcher)
• Family: Protein Kinase/ HGF Receptor/MET
• Target: c-Met
• Immunogen Type: Synthetic peptide
• Immunogen: c-Met antibody was raised against synthetic peptide derived from cytoplasmic domain of human c-Met protein.
• Antigen Modification: Cytoplasmic Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-c-Met antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Related Product Information for anti-MET antibody

Met, an HGF Receptor/Met type protein kinase, is a cell-surface proto-oncogene that mediates the biological activities of hepatocyte growth factor. The protein is a disulfide-linked heterodimer comprised of two subunits: a 50 kD extracellular alpha subunit and a membrane-spanning 145 kD beta subunit that binds ligand. Met is an important regulator of cell proliferation and differentiation, organ regeneration, embryogenesis, and tumorogenesis.

Product Categories/Family for anti-MET antibody

Family: Protein Kinase; Subfamily: HGF Receptor/MET

NCBI and Uniprot Product Information

• NCBI GI #: 42741655
• NCBI GeneID: 4233
• NCBI Accession #: NP_000236.2
• NCBI GenBank Nucleotide #: NM_000245.2
• UniProt Accession #: P08581; O60366; Q12875; Q9UDX7; Q9UPL8; A1L467; B5A932; E7EQ94
• NCBI Official Full Name: hepatocyte growth factor receptor isoform b
• NCBI Official Synonym Full Names: MET proto-oncogene, receptor tyrosine kinase
• NCBI Official Symbol: MET
• NCBI Official Synonym Symbols: HGFR; AUTS9; RCCP2; c-Met
• NCBI Protein Information: hepatocyte growth factor receptor; SF receptor; HGF receptor; HGF/SF receptor; proto-oncogene c-Met; scatter factor receptor; tyrosine-protein kinase Met; met proto-oncogene tyrosine kinase
• UniProt Protein Name: Hepatocyte growth factor receptor
• Protein Family: C-methyltransferase
• UniProt Gene Name: MET
• UniProt Synonym Gene Names: HGF receptor; SF receptor
• UniProt Entry Name: MET_HUMAN

NCBI Description

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Met: a proto-oncogenic receptor tyrosine kinase with high affinity for hepatocyte growth factor. The primary single chain precursor protein is post-translationally cleaved to produce the 45 kDa alpha- and 145 kDa beta-subunits, which are disulfide linked to form the mature receptor. Ligand-binding induces autophosphorylation at multiple tyrosines, which recruit several downstream signaling components, including Gab1, c-Cbl and PI3 kinase. Activating point mutations cause hereditary papillary renal carcinoma. Mutations also seen in sporadic renal cell carcinoma and childhood hepatocellular carcinoma. Upregulation in carcinomas and sarcomas correlates with metastasis and poor outcome. Some gastric carcinomas harbor a translocation that creates an activated TPR-Met fusion protein. A small molecule inhibitor (PHA-665752) shows an effect in gastric carcinoma xenografts. Inhibitors: SU11274, PHA-665752, mAbs. Two alternatively spliced human isoforms have been reported.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.1; Protein kinase, tyrosine (receptor); Membrane protein, integral; Oncoprotein; TK group; Met family

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: cell surface; integral to plasma membrane; basal plasma membrane; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; protein-tyrosine kinase activity; protein phosphatase binding; hepatocyte growth factor receptor activity; ATP binding

Biological Process: axon guidance; skeletal muscle development; peptidyl-tyrosine phosphorylation; activation of MAPK activity; myoblast proliferation; protein amino acid autophosphorylation; myotube differentiation; muscle cell migration; hepatocyte growth factor receptor signaling pathway; glucose homeostasis; liver development; signal transduction; cell proliferation; cell surface receptor linked signal transduction; positive chemotaxis; branching morphogenesis of a tube; adult behavior; positive regulation of transcription from RNA polymerase II promoter; endothelial cell morphogenesis; brain development; placenta development

Disease: Hepatocellular Carcinoma; Renal Cell Carcinoma, Papillary, 1