Mouse anti-Human, Rat ACTH Monoclonal Antibody | anti-ACTH antibody

Mouse Monoclonal [clone 57] (IgG1) to Human ACTH

Cat. Num. AAA245386

• Reactivity: Human, Rat
• Applications: Immunohistochemistry, ELISA
• Purity: Protein A Purified
• Synonyms: ACTH; Monoclonal Antibody; Mouse Monoclonal [clone 57] (IgG1) to Human ACTH; Anti-ACTH Antibody (N-Terminus; clone 57) IHC-plus; Human ACTH; anti-ACTH antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 57
• Specificity: Recognizes human Synacthen (1-24 ACTH). No cross-reactivity to CLIP (17-39 ACTH). Species cross-reactivity: Rat
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.6, 0.09% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 41

• Applicable Applications for anti-ACTH antibody: Immunohistochemistry - Paraffin(IHC-P), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml) (Optimal dilution to be determined by the researcher; Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by pathologist to confirm staining specificity. The optimal working concentration for MBS245386 was determined to be 5 ug/ml.
• Target: ACTH
• Immunogen: ACTH antibody was raised against aCTH (KLH). Epitope: N-terminal
• Antigen Type: Protein
• Epitope: N-terminal
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Upon arrival: Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles

Immunohistochemistry (IHC)

(Anti-ACTH antibody IHC of human pituitary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 490074
• NCBI Official Full Name: ACTH, partial