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Testing Data
(Figure: WB: Recombinant Histone H3 (Lane 1) or acid extracted HeLa cell proteins (Lane 2) were probed with MBS603015 H3 (Lys79) (1:18,000 dilution). Arrow indicates dimethyl-histone H3 (~17kD).)
Rabbit anti-Human Histone H3, dimethyl Monoclonal Antibody | anti-H3 antibody
Histone H3, dimethyl (Lys79)
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Gel Super Shift Assay
Purity
SupernatantSupernatant
Synonyms
Histone H3; dimethyl; Monoclonal Antibody; dimethyl (Lys79); Anti -Histone H3; anti-H3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Clone Number
5H66
Specificity
Recognizes human dimethyl-histone H3 (Lys79) at ~17kD.
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied as a liquid in 0.05% sodium azide.
Applicable Applications for anti-H3 antibody
Western Blot (WB), Immunoprecipitation (IP), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Western Blot, Dot Blot and Histone-Peptide assay. Chromatin Immunoprecipitation (ChIP) has been reported by an independent laboratory.
Dilution: Western Blot: 1:4000 Recombinant Histone H3 or acid extracted HeLa cell proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-14R1. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Peptide Dot Blot Analysis: 1:2500 dilution of this lot detected the immunizing peptide containing dimethyl-lysine 79. Some crossreactivity with peptide containing monomethyl-lysine 79 was also seen. No crossreactivity to other related histone peptides was found. The antibody did not recognize a histone peptide containing the dual modification of dimethyl-lysine 79 and phospho-threonine 80.
Histone-Peptide Specificity Analysis: 1:25-1:1600 dilution of this lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. Peptide containing dimethyl-lysine 79 was readily detected. Some crossreactivity with peptide containing monomethyl-lysine 79 was seen. The antibody did not detect a histone H3 peptide containing the dual modification of dimethyl-lysine 79 and phosphothreonine 80.
Dilution: Western Blot: 1:4000 Recombinant Histone H3 or acid extracted HeLa cell proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-14R1. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system.
Peptide Dot Blot Analysis: 1:2500 dilution of this lot detected the immunizing peptide containing dimethyl-lysine 79. Some crossreactivity with peptide containing monomethyl-lysine 79 was also seen. No crossreactivity to other related histone peptides was found. The antibody did not recognize a histone peptide containing the dual modification of dimethyl-lysine 79 and phospho-threonine 80.
Histone-Peptide Specificity Analysis: 1:25-1:1600 dilution of this lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. Peptide containing dimethyl-lysine 79 was readily detected. Some crossreactivity with peptide containing monomethyl-lysine 79 was seen. The antibody did not detect a histone H3 peptide containing the dual modification of dimethyl-lysine 79 and phosphothreonine 80.
Immunogen
Synthetic peptide ccorresponding to the sequence (...DFme2KTD...), where me2K corresponds to dimethyl-lysine at position 79 of histone H3 conjugated to KLH. The immunizing sequence is identical in human, mouse, rat and yeast.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Figure: WB: Recombinant Histone H3 (Lane 1) or acid extracted HeLa cell proteins (Lane 2) were probed with MBS603015 H3 (Lys79) (1:18,000 dilution). Arrow indicates dimethyl-histone H3 (~17kD).)
Testing Data
(Figure: WB: Recombinant Histone H3 (Lane 1) or acid extracted HeLa cell proteins (Lane 2) were probed with MBS603015 H3 (Lys79) (1:18,000 dilution). Arrow indicates dimethyl-histone H3 (~17kD).)
Dot Blot (DB)
(Peptide Dot Blot Analysis Data:)
Dot Blot (DB)
(Peptide Dot Blot Analysis Data:)
Testing Data
(Beadlyte® Histone-Peptide Specificity Assay Data:)
Testing Data
(Beadlyte® Histone-Peptide Specificity Assay Data:)
Western Blot (WB)
(Western Blotting Analysis:|Representative lot data.|HeLa cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS603015 (1:4000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |Arrow indicates dimethyl Histone H3 (~17kD))
Western Blot (WB)
(Western Blotting Analysis:|Representative lot data.|HeLa cell nuclear extracts were resolved by electrophoresis, transferred to nitrocellulose and probed with MBS603015 (1:4000 dilution). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. |Arrow indicates dimethyl Histone H3 (~17kD))
Related Product Information for anti-H3 antibody
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure.
Product Categories/Family for anti-H3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
NCBI Protein Information
histone H3
Protein Family
Research Articles on H3
Similar Products
Product Notes
The H3 (Catalog #AAA603015) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, dimethyl (Lys79) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3, dimethyl can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Gel Shift Assay (GS/EMSA). Suitable for use in Western Blot, Dot Blot and Histone-Peptide assay. Chromatin Immunoprecipitation (ChIP) has been reported by an independent laboratory. Dilution: Western Blot: 1:4000 Recombinant Histone H3 or acid extracted HeLa cell proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-14R1. Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. Peptide Dot Blot Analysis: 1:2500 dilution of this lot detected the immunizing peptide containing dimethyl-lysine 79. Some crossreactivity with peptide containing monomethyl-lysine 79 was also seen. No crossreactivity to other related histone peptides was found. The antibody did not recognize a histone peptide containing the dual modification of dimethyl-lysine 79 and phospho-threonine 80. Histone-Peptide Specificity Analysis: 1:25-1:1600 dilution of this lot were incubated with histone H3 peptides containing various modifications conjugated to Luminex® microspheres. Peptide containing dimethyl-lysine 79 was readily detected. Some crossreactivity with peptide containing monomethyl-lysine 79 was seen. The antibody did not detect a histone H3 peptide containing the dual modification of dimethyl-lysine 79 and phosphothreonine 80. Researchers should empirically determine the suitability of the H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, dimethyl, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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