Western Blot (WB)
(WWestern Blot analysis using MBS622059 at 0.5ug/ml. Lane 1: Untreated HeLa cell extract Lane 2: HeLa cell treated with sodium butyrate)
Rabbit Histone H3, acetylated Polyclonal Antibody | anti-TTHERM_00189180 antibody
Histone H3, acetylated (Lys9)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Histone H3; acetylated; Polyclonal Antibody; acetylated (Lys9); Anti -Histone H3; anti-TTHERM_00189180 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Histone H3 (Mr 17kD) acetylated at position Lys 9. Species Crossreactivity: mouse, rat and Eukaryote.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide, before the addition of glycerol to 30%.
Applicable Applications for anti-TTHERM_00189180 antibody
Western Blot (WB), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Western and Dot Blot.
Dilution: Western Blot: 1:500. Detects acetylated histone H3 on 10ug of HEK293 lysates.
Dot Blot: 0.5ug/ml. Detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3.
Dilution: Western Blot: 1:500. Detects acetylated histone H3 on 10ug of HEK293 lysates.
Dot Blot: 0.5ug/ml. Detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3.
Immunogen
Synthetic peptide corresponding to aa1-12, ARTKQTAR(AcK)STG-C, where (AcK) denotes acetyl-lysine of human Histone H3, conjugated to KLH. Peptide sequence is identical in a wide range of animal and plant species.
Positive Control
TSA-treated HeLa and NIH/3T3 cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WWestern Blot analysis using MBS622059 at 0.5ug/ml. Lane 1: Untreated HeLa cell extract Lane 2: HeLa cell treated with sodium butyrate)
Western Blot (WB)
(WWestern Blot analysis using MBS622059 at 0.5ug/ml. Lane 1: Untreated HeLa cell extract Lane 2: HeLa cell treated with sodium butyrate)
Western Blot (WB)
(Western Blot analysis of HEK293 lysate, resolved by electrophoresis, transferred to membrane and probed with MBS622059 (1:500). Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates Histone H3, acetylated (Lys9) (~17kD).)
Western Blot (WB)
(Western Blot analysis of HEK293 lysate, resolved by electrophoresis, transferred to membrane and probed with MBS622059 (1:500). Proteins were visualized using an IgG (HRP) donkey anti-rabbit secondary antibody and a chemiluminescence detection system. Arrow indicates Histone H3, acetylated (Lys9) (~17kD).)
Western Blot (WB)
(Western Blot analysis was resolved by electrophoresis, transferred to PVDF membrane and using MBS622059 (1:500 dilution). Lane1: A431, Lane2: C2C12 lysate, Lane 3: HEK293 lysate, Lane 4: Hela lysate, Lane 5: HepG2 lysate, Lane 6: Jurkat lysate, Lane 7: L6 lysate, Lane 8: NIH/3T3 lysate)
Western Blot (WB)
(Western Blot analysis was resolved by electrophoresis, transferred to PVDF membrane and using MBS622059 (1:500 dilution). Lane1: A431, Lane2: C2C12 lysate, Lane 3: HEK293 lysate, Lane 4: Hela lysate, Lane 5: HepG2 lysate, Lane 6: Jurkat lysate, Lane 7: L6 lysate, Lane 8: NIH/3T3 lysate)
Related Product Information for anti-TTHERM_00189180 antibody
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Acetylation of histone H3 occurs at several different lysine positions in the histone tail and is performed by a family of enzymes known as Histone Acetyl Transferases (HATs). Acetylation of lysine14 is commonly seen in genes that are being actively transcribed into RNA.
Product Categories/Family for anti-TTHERM_00189180 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The TTHERM_00189180 (Catalog #AAA622059) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, acetylated (Lys9) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3, acetylated can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Gel Shift Assay (GS/EMSA). Suitable for use in Western and Dot Blot. Dilution: Western Blot: 1:500. Detects acetylated histone H3 on 10ug of HEK293 lysates. Dot Blot: 0.5ug/ml. Detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3. Researchers should empirically determine the suitability of the TTHERM_00189180 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, acetylated, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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