Western Blot (WB)
(Peptide Inhibition: Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.)
Mouse anti-Human Histone H3, monomethyl Monoclonal Antibody | anti-H3 antibody
Histone H3, monomethyl (Lys9)
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Histone H3; monomethyl; Monoclonal Antibody; monomethyl (Lys9); Anti -Histone H3; anti-H3 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
10A19
Specificity
Recognizes human monomethylated Lys9 at and around the N-terminal of Histone H3, MW ~17kD. Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.05% sodium azide.
Applicable Applications for anti-H3 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot and Peptide Inhibition Assay.
Dilution: Western Blot: 1ug/ml detects Histone H3 in 500ng of recombinant Histone H3 and 10ug of HeLa acid extract.
Dilution: Western Blot: 1ug/ml detects Histone H3 in 500ng of recombinant Histone H3 and 10ug of HeLa acid extract.
Immunogen
Linear peptide corresponding to monomethylated Lys9 at the N-terminal region of human Histone H3, conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Peptide Inhibition: Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.)
Western Blot (WB)
(Peptide Inhibition: Specificity confirmed by the ability of immunizing peptide to abolish detection of monomethyl Histone H3 (Lys9) (Lane 7) in Western Blot analysis of HeLa acid extracts and various other modified histone peptides.)
Western Blot (WB)
(Western Blot: Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS608628 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Western Blot (WB)
(Western Blot: Recombinant Histone H3 (Lane 1) and HeLa acid extract (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with MBS608628 (1ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.)
Testing Data
Testing Data
Related Product Information for anti-H3 antibody
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine. Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. Also weakly methylates Histone H1 (in vitro). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as repression of MYOD1-stimulated differentiation, regulation of the control switch for exiting the cell cycle and entering differentiaton, repression by the PML-RARA fusion protein, BMP-induced repression, repression of switch recombination to IgA and regulation of telomere length.
Product Categories/Family for anti-H3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
NCBI Protein Information
histone H3
Protein Family
Research Articles on H3
Similar Products
Product Notes
The H3 (Catalog #AAA608628) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, monomethyl (Lys9) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3, monomethyl can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot and Peptide Inhibition Assay. Dilution: Western Blot: 1ug/ml detects Histone H3 in 500ng of recombinant Histone H3 and 10ug of HeLa acid extract. Researchers should empirically determine the suitability of the H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, monomethyl, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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