Mouse Histones, pan Monoclonal Antibody

Histones, pan

Cat. Num. AAA600046

• Reactivity: bovine, rat, Xenopusm opossum, hamster.
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Histones; pan; Monoclonal Antibody; Anti -Histones; anti-Histones; pan antibody

• Host: Mouse
• Reactivity: bovine, rat, Xenopusm opossum, hamster.
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 2Q2283
• Specificity: Recognizes human histones HI1, H2A, h2B, H3 and H4 with comparable sensitivity. Binds specifically to an antigen determinant, which is present on all five histone proteins.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in 0.02M PBS, pH 7.6, 0.09% sodium azide.
• Concentration: 1 mg/mL (varies by lot)

• Applicable Applications for anti-Histones, pan antibody: Suitable for use in Immunohistochemistry, Western Blot and Immunocytochemistry. Other applications have not been tested.
• Application Notes: Immunohistochemistry: 1-5 ug/mL; Western Blot: Detects a band ~20kD; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Purified nuclear fractions of HeLa cells
• Preparation and Storage: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat lysate (Lane 1) & mouse brain lysate (Lane 2) were resolved by electrophoresis, transferred to PVDF membrane and probed with H3505 (1:500).|Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.|Arrow indicates protein Histone (Pan) (~20kD).)

Western Blot (WB)

(Western Blot analysis in Jurkat lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with MBS600046 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.|Arrow indicates protein Histone (Pan) (~20kD).)

Immunofluorescence (IF)

(Confocal fluorescent analysis of NIH/3T3 using MBS600046 mouse mAB(blue). Actin filaments have been labeled with Rhodamine Phalloidin(red).)

Related Product Information for anti-Histones, pan antibody

There are 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail histones are involved with the structure of the nucleosomes of the 'beads on a string' structure.

Product Categories/Family for anti-Histones, pan antibody

Antibodies; Abs to Histones