Typical Testing Data/Standard Curve (for reference only)
Mouse Insulin Autoantibody (IAA) ELISA Kit | IAA elisa kit
Mouse Insulin Autoantibody (IAA) ELISA Kit
Reactivity
Mouse
Applications
ELISA
Synonyms
Insulin Autoantibody (IAA); Mouse Insulin Autoantibody (IAA) ELISA Kit; Anti-Insulin Antibody; Antiinsulin; Insulin Antibodies; IAA elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of IAA. No significant cross-reactivity or interference between IAA and analogues was observed.
Applicable Applications for IAA elisa kit
ELISA (EIA)
Application Notes
Enzyme-linked Immunosorbent Assay for Antibody Detection.
Samples
Serum, plasma, tissue homogenatesand other biological fluids
Detection Range
3.12-200ng/mL
Sensitivity
< 1.18ng/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IAA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IAA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IAA elisa kit
Intended Uses: The kit is an enzyme immunoassay for in vitro quantitative measurement of IAA in mouse serum, plasma, tissue homogenatesand other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with IAA. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain IAA will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with IAA. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain IAA will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of IAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Similar Products
Product Notes
The Mouse IAA (Catalog #AAA2124023) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2124023 ELISA Kit recognizes Mouse IAA. AAA Biotech's Insulin Autoantibody (IAA) can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Enzyme-linked Immunosorbent Assay for Antibody Detection. Researchers should empirically determine the suitability of the IAA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Autoantibody (IAA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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