Western Blot (WB)
(WB: Recombinant histone H3 (H5110-12K, lane 1) and chicken core histones (H5110-10A, lane 2) were probed with MBS617174 -Histone H3 (Lys36) (1:1000).)
Rabbit anti-Human Histone H3, monomethyl Polyclonal Antibody | anti-H3 antibody
Histone H3, monomethyl (Lys36)
Reactivity
Human
Applications
Western Blot, Immunofluorescence, Gel Super Shift Assay
Purity
SerumSerum
Synonyms
Histone H3; monomethyl; Polyclonal Antibody; monomethyl (Lys36); Anti -Histone H3; anti-H3 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human monomethyl-Histone H3 (Lys36), Mr ~17kD. A non-specific protein was also detected, Mr ~200kD. Broad species crossreactivity is expected.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid in 0.05% sodium azide, 30% glycerol.
Applicable Applications for anti-H3 antibody
Western Blot (WB), Immunofluorescence (IF), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Western Blot, Immunofluorescence, Beadlyte Histone Peptide Assay and Peptide Dot Blot Analysis.
Dilution: Western Blot: 1:500-1:2000 detected monomethyl-Histone H3 (Lys36) in chicken core histones. Did not detect unmodified recombinant Histone H3. Recombinant histone H3 and chicken core histones were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-11N. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Beadlyte Histone Peptide Assay: 1:10,000-1:80,000 detected only the histone H3 peptide containing the monomethyl lysine 36 modification
Peptide Dot Blot Analysis: 1:5000 detected only the histone H3 peptide containing the monomethyl lysine 36 modification. A dilution series of Histone H3 peptides.
Dilution: Western Blot: 1:500-1:2000 detected monomethyl-Histone H3 (Lys36) in chicken core histones. Did not detect unmodified recombinant Histone H3. Recombinant histone H3 and chicken core histones were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-11N. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Beadlyte Histone Peptide Assay: 1:10,000-1:80,000 detected only the histone H3 peptide containing the monomethyl lysine 36 modification
Peptide Dot Blot Analysis: 1:5000 detected only the histone H3 peptide containing the monomethyl lysine 36 modification. A dilution series of Histone H3 peptides.
Immunogen
Synthetic peptide containing the sequence …GVmeKKP… in which meK corresponds to monomethyl Lysine 36 of human histone H3, conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(WB: Recombinant histone H3 (H5110-12K, lane 1) and chicken core histones (H5110-10A, lane 2) were probed with MBS617174 -Histone H3 (Lys36) (1:1000).)
Western Blot (WB)
(WB: Recombinant histone H3 (H5110-12K, lane 1) and chicken core histones (H5110-10A, lane 2) were probed with MBS617174 -Histone H3 (Lys36) (1:1000).)
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for anti-H3 antibody
Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eurokaryotic cells. Featuring a main globuular domaina and a long N-terminal tail. H3 is involved with the structure of the nucleosomes of the "beads of string" structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
Product Categories/Family for anti-H3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
histone H3
NCBI Official Symbol
H3
NCBI Protein Information
histone H3
Protein Family
Research Articles on H3
Similar Products
Product Notes
The H3 (Catalog #AAA617174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3, monomethyl (Lys36) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3, monomethyl can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Gel Shift Assay (GS/EMSA). Suitable for use in Western Blot, Immunofluorescence, Beadlyte Histone Peptide Assay and Peptide Dot Blot Analysis. Dilution: Western Blot: 1:500-1:2000 detected monomethyl-Histone H3 (Lys36) in chicken core histones. Did not detect unmodified recombinant Histone H3. Recombinant histone H3 and chicken core histones were resolved by electrophoresis, transferred to nitrocellulose and probed with H5110-11N. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Beadlyte Histone Peptide Assay: 1:10,000-1:80,000 detected only the histone H3 peptide containing the monomethyl lysine 36 modification Peptide Dot Blot Analysis: 1:5000 detected only the histone H3 peptide containing the monomethyl lysine 36 modification. A dilution series of Histone H3 peptides. Researchers should empirically determine the suitability of the H3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3, monomethyl, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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