Monkey Lactoferrin ELISA Kit | LF elisa kit

Monkey Lactoferrin ELISA Kit

Cat. Num. AAA743082

• Gene Names: LTF; LF; HLF2; GIG12; HEL110
• Reactivity: Monkey
• Synonyms: Lactoferrin; Monkey Lactoferrin ELISA Kit; LF elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 2.5-50ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This LF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey LF. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LF elisa kit

Principle of the Assay: LF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LF antibody and an LF-HRP conjugate. The assay sample and buffer are incubated together with LF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LF concentration since LF from samples and LF-HRP conjugate compete for the anti-LF antibody binding site. Since the number of sites is limited, as more sites are occupied by LF from the sample, fewer sites are left to bind LF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LF concentration in each sample is interpolated from this standard curve.

Product Categories/Family for LF elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 187122
• NCBI GeneID: 4057
• Molecular Weight: 73,161 Da
• NCBI Official Full Name: lactoferrin
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: LF; HLF2; GIG12; HEL110
• NCBI Protein Information: lactotransferrin; talalactoferrin; neutrophil lactoferrin; growth-inhibiting protein 12; epididymis luminal protein 110
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactoferrin-binding protein
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: GIG12; LF; Lactoferrin; Lfcin-H
• UniProt Entry Name: TRFL_HUMAN

NCBI Description

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2010]

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.21.-; Secreted, signal peptide; Protease; Secreted

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule

Molecular Function: heparin binding; protein serine/threonine kinase activator activity; protein binding; DNA binding; ferric iron binding; iron ion binding; serine-type endopeptidase activity

Biological Process: interaction with host; negative regulation of lipopolysaccharide-mediated signaling pathway; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; iron ion transport; humoral immune response; regulation of cytokine production; activation of NF-kappaB transcription factor; retinal homeostasis; positive regulation of osteoblast differentiation; negative regulation of viral genome replication; innate immune response in mucosa; response to host immune response; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; negative regulation of ATPase activity; negative regulation of viral reproduction; iron assimilation by chelation and transport; negative regulation of apoptosis