Western Blot (WB)
(Western Blot analysis in mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS632847 (0.5ug/ml). Proteins were visualized using an IgG (HRP) Goat anti-mouse and chemiluminescence detection system. Arrow indicates Glutamate/Aspartate Transporter 2 (~62kD).)
Mouse anti-Human, Mouse Glutamate/Aspartate Transporter 2 Monoclonal Antibody
Glutamate/Aspartate Transporter 2 (GLT1, EAAT2, Excitatory Amino Acid Transporter 2)
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
Glutamate/Aspartate Transporter 2; Monoclonal Antibody; Glutamate/Aspartate Transporter 2 (GLT1; EAAT2; Excitatory Amino Acid Transporter 2); Anti -Glutamate/Aspartate Transporter 2 (GLT1; anti-Glutamate/Aspartate Transporter 2 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1k
Clone Number
10H26
Specificity
Recognizes human Glutamate/Aspartate Transporter 2 at ~62kD. Species Crossreactivity: Mouse.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Applicable Applications for anti-Glutamate/Aspartate Transporter 2 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 0.5ug/ml detected Glutamate/Aspartate Transporter 2 on 10ug of mouse brain tissue lysate.
Dilution: Western Blot: 0.5ug/ml detected Glutamate/Aspartate Transporter 2 on 10ug of mouse brain tissue lysate.
Immunogen
Linear peptide corresponding to the C-terminus of human Glutamate/Aspartate Transporter 2 (NP_004162). Species Sequence Homology: Rat.
Positive Control
Mouse brain tissue lysate
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS632847 (0.5ug/ml). Proteins were visualized using an IgG (HRP) Goat anti-mouse and chemiluminescence detection system. Arrow indicates Glutamate/Aspartate Transporter 2 (~62kD).)
Western Blot (WB)
(Western Blot analysis in mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with MBS632847 (0.5ug/ml). Proteins were visualized using an IgG (HRP) Goat anti-mouse and chemiluminescence detection system. Arrow indicates Glutamate/Aspartate Transporter 2 (~62kD).)
Related Product Information for anti-Glutamate/Aspartate Transporter 2 antibody
The activity of Glutamate/Aspartate Transporter 2 (also known as GLT1), the main glutamate transporter in the CNS, can be regulated by protein kinase C (PKC). It is known that activation of PKC by phorbol esters promotes the clathrin-dependent internalization of the transporter, followed by its lysosomal degradation. However, the molecular mechanisms that link PKC activation and the internalization of the Glutamate/Aspartate Transporter 2 are not fully understood. The internalization process may play a role in the ubiquitylation of lysine residues located in the C-terminal tail of the Glutamate/Aspartate Transporter 2. Exposure to PMA increases the ubiquitylation of the Glutamate/Aspartate Transporter 2 in transfected cells and in the rat brain, and this ubiquitylated Glutamate/Aspartate Transporter 2 accumulates in the intracellular compartment. The activation of PKC induces the ubiquitylation of these C-terminal lysine residues in the Glutamate/Aspartate Transporter 2 and this modification mediates the interaction of the transporter with the endocytic machinery.
Product Categories/Family for anti-Glutamate/Aspartate Transporter 2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
glutamate/ aspartate transporter
Similar Products
Product Notes
The Glutamate/Aspartate Transporter 2 (Catalog #AAA632847) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Glutamate/Aspartate Transporter 2 (GLT1, EAAT2, Excitatory Amino Acid Transporter 2) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamate/Aspartate Transporter 2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot: 0.5ug/ml detected Glutamate/Aspartate Transporter 2 on 10ug of mouse brain tissue lysate. Researchers should empirically determine the suitability of the Glutamate/Aspartate Transporter 2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamate/Aspartate Transporter 2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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